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Scalable Transfection of Maize Mesophyll Protoplasts
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An Efficient Cell Sorting Protocol for Maize Protoplasts.

Carlos Ortiz-Ramírez1, Edgar Demesa Arevalo2, Xiaosa Xu2

  • 1Center for Genomics and Systems Biology, Department of Biology, New York University, New York, New York.

Current Protocols in Plant Biology
|August 24, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces an improved method for isolating maize protoplasts, significantly increasing yield from inner tissues. This advancement facilitates advanced transcriptomic analysis in maize and other plant species.

Keywords:
FACSmaizemonocotsprotoplaststranscriptome

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Area of Science:

  • Plant Science
  • Genetics
  • Molecular Biology

Background:

  • Maize is a vital crop and model organism for genetic studies.
  • Transcriptomic analysis requires efficient isolation of specific cell types.
  • Existing protoplast isolation methods are insufficient for inner maize tissues.

Purpose of the Study:

  • To develop an improved protocol for high-yield protoplast isolation from maize.
  • To enable fluorescence-activated cell sorting (FACS) based transcriptomic studies in maize.

Main Methods:

  • Tissue digestion and cell wall removal to generate protoplasts.
  • Optimization of enzymatic treatment for enhanced protoplast yield.
  • Application of the protocol to maize root and shoot tissues.

Main Results:

  • The new protocol dramatically increases protoplast yield from maize.
  • Efficient protoplast generation from inner tissue layers was achieved.
  • The protocol demonstrated success in both maize and Arabidopsis thaliana.

Conclusions:

  • This optimized protocol significantly enhances protoplast isolation efficiency in maize.
  • It provides a robust method for cell-type-specific transcriptomic analysis in maize.
  • The protocol is adaptable for use in other plant species, including Arabidopsis.