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Long Noncoding RNA EGFR-AS1 Promotes Cell Proliferation by Increasing EGFR mRNA Stability in Gastric Cancer

Jiaojiao Hu1, Yingying Qian2, Lipan Peng3

  • 1Nanjing Maternity and Child Health Care Institute, Nanjing Maternity and Child Health Care Hospital, Obsterics and Gynecology Hospital Affiliated to Nanjing Medical University, Nanjing, China.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
|August 24, 2018
PubMed
Abstract

Insights

Long non-coding RNA EGFR-AS1 promotes gastric cancer (GC) progression by increasing EGFR expression and stabilizing its mRNA. Silencing EGFR-AS1 inhibits GC cell proliferation, suggesting it as a potential GC therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Gene Expression Regulation

Background:

  • Gastric cancer (GC) is a significant health concern.
  • Long non-coding RNA (lncRNA) EGFR-AS1 is implicated in GC progression.
  • EGFR-AS1 acts as an antisense transcript to the Epidermal Growth Factor Receptor (EGFR).

Purpose of the Study:

  • To investigate the role of lncRNA EGFR-AS1 in gastric cancer.
  • To elucidate the underlying mechanisms by which EGFR-AS1 influences GC.
  • To assess EGFR-AS1 as a potential therapeutic target for GC.

Main Methods:

  • Silencing EGFR-AS1 expression using shRNA lentivirus in GC cell lines (MGC803, SGC-7901).
  • Quantification of lncRNA EGFR-AS1 and EGFR mRNA/protein levels via qPCR and Western Blot.
  • Assessment of cell proliferation using CCK-8, EdU, and colony formation assays.
  • Investigation of EGFR mRNA stability using the RNA synthesis inhibitor α-amanitin.

Main Results:

  • EGFR-AS1 was significantly upregulated in GC tissues and correlated with tumor size.
  • EGFR-AS1 expression positively correlated with EGFR expression in GC tissues.
  • Knockdown of EGFR-AS1 inhibited GC cell proliferation in vitro and in vivo.
  • EGFR-AS1 depletion reduced EGFR expression by decreasing EGFR mRNA stability, impacting the PI3K/AKT pathway.

Conclusions:

  • lncRNA EGFR-AS1 exhibits oncogenic properties in gastric cancer.
  • EGFR-AS1 may promote GC progression through EGFR-dependent pathways.
  • EGFR-AS1 represents a potential therapeutic target for gastric cancer treatment.
Keywords:
EGFREGFR-AS1Gastric cancerMRNA stabilityPI3KlncRNAs

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