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Live Intracellular Biorthogonal Imaging by Surface Enhanced Raman Spectroscopy using Alkyne-Silver Nanoparticles
Matteo Ardini1, Jian-An Huang1, Carlos S Sánchez2
1Istituto Italiano di Tecnologia, Via Morego 30, 16163, Genova, Italy.
Scientific Reports
|August 25, 2018
Summary
Researchers developed novel Surface Enhanced Raman Spectroscopy (SERS) probes for clear intracellular imaging. These probes detect dopamine within cells, overcoming background noise for enhanced diagnostics and drug development.
Area of Science:
- Biomedical Engineering
- Spectroscopy
- Nanotechnology
Background:
- Live intracellular imaging is crucial for diagnostics and pharmacology.
- Surface Enhanced Raman Spectroscopy (SERS) offers non-destructive, multiplexed imaging.
- Current intracellular SERS faces challenges with endogenous background interference.
Purpose of the Study:
- To develop SERS probes for biorthogonal intracellular imaging of dopamine.
- To overcome limitations of endogenous background noise in SERS.
- To enable real-time imaging of biomolecules within living cells.
Main Methods:
- Assembly of small colloidal SERS probes using silver nanoparticles.
- Linking nanoparticles with alkyne-dopamine adducts to form clusters.
- Utilizing the cell-silent Raman window (1800-2900 cm-1) for signal detection.
Main Results:
- Achieved high electric field enhancement and strong SERS signals from alkyne and dopamine.
- Demonstrated distinguishable dopamine imaging within the cytoplasm with good spatial resolution.
- Successfully minimized interference from endogenous cellular components.
Conclusions:
- The developed SERS probes enable undistinguishable intracellular dopamine imaging.
- This method provides a general-purpose platform for real-time biomolecule imaging.
- The approach holds potential for advancements in diagnostics and pharmacology.
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