Duplex microRNAs assay based on target-triggered universal reporter hybridization
Yinan Wang1, Yue Sun1, Choiwan Lau1
1School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai 201203, China.
Journal of Pharmaceutical Analysis
|August 25, 2018
Summary
This study introduces a novel duplex detection strategy for microRNAs (miRNAs) using target-triggered hybridization. This method enables sensitive and selective detection of multiple miRNA biomarkers for disease diagnosis.
Area of Science:
- Biotechnology
- Molecular Biology
- Assay Development
Background:
- MicroRNAs (miRNAs) are crucial biomarkers for disease diagnosis and prognosis.
- Accurate and sensitive detection of multiple miRNAs is essential for clinical applications.
Purpose of the Study:
- To design and evaluate a duplex detection strategy for microRNA detection.
- To develop a highly selective and sensitive assay for multiple miRNA biomarkers.
Main Methods:
- Utilized a universal probe-based, target-triggered double hybridization approach.
- Employed a fluorescent microsphere-based assay system (xMAP array) for detection.
Main Results:
- Achieved quantitative detection of duplex miRNAs with a limit of detection as low as 40 pM.
- Demonstrated high selectivity and sensitivity due to target-triggered stable hybridization.
- The xMAP array platform shows potential for simultaneous detection of up to 100 miRNA targets.
Conclusions:
- The developed method offers a simple, rapid, and multiplexing approach for miRNA detection.
- This strategy holds promise for the detection of multiple miRNA biomarkers for early disease diagnosis and prognosis.
Related Concept Videos
01:22MicroRNAs
24.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.2K
01:22MicroRNAs
4.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
02:29Hybrid Zones
21.9K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.9K
03:24Hybridization of Atomic Orbitals I
67.6K
The mathematical expression known as the wave function, ψ, contains information about each orbital and the wavelike properties of electrons in an isolated atom. When atoms are bound together in a molecule, the wave functions combine to produce new mathematical descriptions that have different shapes. This process of combining the wave functions for atomic orbitals is called hybridization and is mathematically accomplished by the linear combination of atomic orbitals. The new orbitals that...
67.6K
03:35Hybridization of Atomic Orbitals II
49.2K
sp3d and sp3d 2 Hybridization
49.2K
01:24Data Reporting and Recording
5.5K
Reporting and recording are crucial in data documentation. The timely, thorough, and accurate documentation of facts is essential when recording patient data. Failure to record findings during an assessment or interpretation of a problem will result in loss of information and make the patient document unreliable. The reader is left with general impressions if the information is not specific. A recording is documenting data of the individual's health information in a traceable, secure, and...
5.5K