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Related Experiment Video

Updated: Feb 6, 2026

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

3D Microtissues for Injectable Regenerative Therapy and High-throughput Drug Screening

Published on: October 4, 2017

A high-throughput microtissue platform to probe endothelial function in vitro.

Alexandra L Crampton¹, Katherine A Cummins, David K Wood

¹Department of Biomedical Engineering, University of Minnesota, Twin Cities, USA. dkwood@umn.edu.

Integrative Biology : Quantitative Biosciences From Nano to Macro

|August 25, 2018

Summary

This summary is machine-generated.

Researchers developed a scalable microfluidic platform to assess endothelial barrier function using extracellular matrix (ECM) microtissues. This high-throughput method accurately measures endothelial permeability, mimicking in vivo conditions for drug discovery and research.

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Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Assessing Functional Metrics of Skeletal Muscle Health in Human Skeletal Muscle Microtissues

Published on: February 18, 2021

Area of Science:

Biomedical Engineering
Cell Biology
Vascular Biology

Background:

The vascular endothelium acts as a semi-permeable barrier, crucial for regulating solute exchange between blood and tissues.
Current in vitro models for endothelial function are limited by low throughput or lack of physiologically relevant extracellular matrix (ECM) interactions.
Accurate assessment of endothelial barrier function is vital for understanding disease and developing therapeutics.

Purpose of the Study:

To develop a scalable, high-throughput microfluidic platform for measuring endothelial function in ECM-based microtissues.
To incorporate physiologically relevant cell-ECM interactions into in vitro endothelial models.
To assess endothelial permeability and its response to modulators in a high-throughput format.

Main Methods:

Fabrication of endothelial-coated collagen microtissues using droplet microfluidics.
Characterization of endothelial cell morphology, tight junction protein expression, and ECM remodeling.
Quantification of macromolecular permeability using optical modalities and assessment of responses to TNF-α and TGF-β.

Main Results:

The microtissues demonstrated characteristic endothelial cell morphology and tight junction protein expression.
Endothelial cells remodeled the ECM through compaction and basement membrane deposition.
Measured permeability values were comparable to in vivo data and responsive to inflammatory modulators (TNF-α, TGF-β).

Conclusions:

This novel microfluidic platform enables high-throughput assessment of endothelial permeability on natural ECM, surpassing limitations of traditional methods.
The platform accurately recapitulates in vivo endothelial barrier function and interactions with the ECM.
This technology offers a scalable solution for interrogating endothelial cell-matrix and cell-cell interactions, with potential applications in drug screening and disease modeling.