Dual-Porosity Hollow Carbon Spheres with Tunable Through-Holes for Multi-Guest Delivery

Dual-porosity hollow carbon spheres (DPHCs) with small mesopores (2-4 nm) and large through-holes (20-30 nm) in shells were successfully synthesized using colloidal silica as the template, small silica nanoparticles as nanomasks, and nontoxic dopamine as the carbon precursor followed by post-carbonization and etching. The synthesized DPHCs were further oxidized to be hydrophilic and then used to simultaneously deliver the protein bovine serum albumin (21 × 4 × 14 nm³) and the small molecule doxorubicin (<1 nm), which exhibited a high loading capacity of 689.4 and 1421.2 mg/g, respectively. The release of these two guest molecules can be controlled independently under the stimuli of heat and acidity. In vitro and in vivo experiments also proved that the DPHCs are promising for the co-delivery of multiple cargoes of different sizes.