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The Microtubule-Associated Protein CLASP Sustains Cell Proliferation through a Brassinosteroid Signaling Negative

Yuan Ruan1, Laryssa S Halat1, Deirdre Khan2

  • 1Department of Botany, The University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T 1Z4, Canada.

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|August 28, 2018
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Summary

Plant meristem cell division relies on CLASP protein, which is negatively regulated by brassinosteroid signaling. CLASP sustains this signaling by recycling receptors, revealing a feedback loop for meristem stability.

Keywords:
BRI1CLASPauxinbrassinosteroidcell divisionhormone signalingmicrotubuleplant meristemretromersorting nexin 1

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Area of Science:

  • Plant Biology
  • Cell Biology
  • Molecular Biology

Background:

  • Plant meristematic capacity is crucial for organ development and function.
  • Mutations in CLASP (microtubule-associated protein) and increased brassinosteroid signaling impair meristem cell division.

Purpose of the Study:

  • To investigate the interplay between CLASP and brassinosteroid signaling in Arabidopsis root apical meristems.
  • To elucidate the molecular mechanisms underlying meristem homeostasis.

Main Methods:

  • Analysis of CLASP transcript and protein levels under altered brassinosteroid signaling.
  • Microtubule organization assessment.
  • Cell counting in meristems.
  • Investigating CLASP's role in BRI1 receptor trafficking.
  • Transcript profiling of clasp-1 mutants.

Main Results:

  • Enhanced brassinosteroid signaling reduces CLASP levels and disrupts microtubule organization, leading to fewer meristem cells.
  • CLASP facilitates brassinosteroid signaling by promoting the retrieval of endocytosed BRI1 receptors to the plasma membrane.
  • clasp-1 mutants exhibit reduced brassinosteroid-mediated transcriptional activity and responses.
  • Transcript profiling reveals impaired cell-cycle activity and hormone crosstalk in clasp-1 mutants.

Conclusions:

  • CLASP is a key regulator targeted by brassinosteroid signaling and also promotes it.
  • CLASP sustains meristem homeostasis through a negative feedback loop involving brassinosteroid receptor trafficking.
  • This study uncovers a novel mechanism of hormone crosstalk essential for plant development.