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Related Concept Videos

Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

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Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
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Precipitation Titration Curve: Analysis01:21

Precipitation Titration Curve: Analysis

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The precipitation titration curve demonstrates the change in concentration of one reactant with the volume of titrant added. During the titration of chloride ions with silver nitrate, the precipitation titration curve is divided into three regions: before, at, and after the equivalence point. Before the equivalence point, low redissolution of the sparingly soluble silver chloride precipitate gives a low silver ion concentration. However, in the second region, representing the equivalence point,...
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Bending of Curved Members - Strain Analysis01:14

Bending of Curved Members - Strain Analysis

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The mechanics of deformation in curved members, such as beams or arches, under bending moments, involve complex responses. When such a member, symmetric about the y-axis and shaped like a segment of a circle centered at point C, is subjected to equal and opposite forces, its curvature and surface lengths change significantly. This alteration results in the shift of the curvature's center from C to C', indicating a tighter curve.
The important part of bending analysis for such a member...
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Sieve Analysis and Grading Curves01:19

Sieve Analysis and Grading Curves

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Sieve analysis is a method used to determine the particle size distribution of aggregate materials. This process involves the following steps:
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Heating and Cooling Curves02:44

Heating and Cooling Curves

28.0K
When a substance—isolated from its environment—is subjected to heat changes, corresponding changes in temperature and phase of the substance is observed; this is graphically represented by heating and cooling curves.
For instance, the addition of heat raises the temperature of a solid; the amount of heat absorbed depends on the heat capacity of the solid (q = mcsolidΔT). According to thermochemistry, the relation between the amount of heat absorbed or released by a substance, q, and its...
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Acid-Base Titration Curves02:23

Acid-Base Titration Curves

141.5K
A titration curve is a plot of some solution property versus the amount of added titrant. For acid-base titrations, solution pH is a useful property to monitor because it varies predictably with the solution composition and, therefore, may be used to monitor the titration’s progress and detect its endpoint. Acid-base titration can be performed with a strong acid and a strong base, a strong acid and a weak base, or a strong base and a weak acid.
For a titration carried out for 25.00 mL of...
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Related Experiment Video

Updated: Feb 6, 2026

Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis
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Rapid and Efficient Zebrafish Genotyping Using PCR with High-resolution Melt Analysis

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High-Throughput and Rapid Melting Curve Analysis.

Yan-Long Hou, Chong-Ge You

    Clinical Laboratory
    |August 28, 2018
    PubMed
    Summary
    This summary is machine-generated.

    A novel microfluidic-based platform enhances high-resolution melting curve analysis (HRM) for genetic mutation detection. This integrated system offers high-throughput, rapid, portable, and cost-effective genotyping and scanning.

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    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Genetics

    Background:

    • High-resolution melting curve analysis (HRM) is a key technology for genetic mutation scanning.
    • Integration of PCR amplification and HRM detection has spurred interest in improving throughput and speed.
    • Existing methods face challenges in achieving high-throughput, rapid, and portable genetic analysis.

    Purpose of the Study:

    • To develop a novel high-throughput PCR-HRM detection mode.
    • To integrate microfluidic technology with PCR-HRM for enhanced genetic analysis.
    • To address the need for portable and cost-effective genetic scanning solutions.

    Main Methods:

    • Systematic literature search of PubMed and ScienceDirect for microfluidic and HRM technologies.
    • Focus on microfluidic device manufacture and applications in medical diagnostics.
    • Review of references from identified articles.

    Main Results:

    • A new high-throughput PCR-HRM genotyping and scanning detection mode was developed.
    • Microfluidic integration enables closed-tube, high-throughput, and rapid detection.
    • The system demonstrates portability and cost-effectiveness.

    Conclusions:

    • The microfluidic-based PCR-HRM platform achieves high throughput.
    • This technology offers high-speed genetic detection capabilities.
    • The platform meets demands for portability and affordability in genetic analysis.