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Updated: Feb 6, 2026
A Fast and Quantitative Method for Post-translational Modification and Variant Enabled Mapping of Peptides to Genomes
Published on: May 22, 2018
Proteomics of Pseudomonas aeruginosa: the increasing role of post-translational modifications
Charlotte Gaviard1,2, Thierry Jouenne1,2, Julie Hardouin1,2
1a Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS , 76000 , Rouen , France.
Introduction:
Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen widely involved in human infections. The high occurrence of this bacterial species in the clinical field is due to its high ability to adapt to detrimental environments, in particular its strong inherent antibiotic resistance, its ability to form biofilms and to produce virulence factors. The application of proteomics to clinical microbiology is probably one of the most innovative strategies of the last decades to understand complex microbial systems, by providing individual proteome charts of pathogens. Areas covered: In the last decade, proteomic advances have allowed in high-throughput the screening of proteins modified by diverse co- and post-translational modifications in P. aeruginosa. This review will present the current state of the art for the characterization of PTMs in P. aeruginosa by proteomics approaches. We will then discuss on the involvement of PTMs in P. aeruginosa physiology. Expert commentary: Modified proteins and enzymes involved in the addition/removal of modifications will surely constitute targets of interest to develop new therapeutic drugs to fight against P. aeruginosa.
Insights
Pseudomonas aeruginosa, a resilient pathogen, exhibits antibiotic resistance and biofilm formation. Proteomics reveals post-translational modifications (PTMs) crucial for its survival, offering new therapeutic targets.
Area of Science:
- Microbiology
- Proteomics
- Biochemistry
Background:
- Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen known for its adaptability and resistance to antibiotics.
- Its ability to form biofilms and produce virulence factors contributes to persistent human infections.
- Proteomics offers a powerful approach to understand complex microbial systems by analyzing pathogen proteomes.
Purpose of the Study:
- To review the current state of characterizing post-translational modifications (PTMs) in P. aeruginosa using proteomics.
- To discuss the role of PTMs in P. aeruginosa physiology and adaptation.
- To highlight the potential of PTMs as therapeutic targets.
Main Methods:
- High-throughput proteomic screening of modified proteins in P. aeruginosa.
- Analysis of co- and post-translational modifications.
- Review of existing literature on PTMs in P. aeruginosa.
Main Results:
- Proteomic advances have enabled high-throughput screening of PTMs in P. aeruginosa.
- PTMs play a significant role in P. aeruginosa physiology and adaptation.
- Identification of modified proteins and associated enzymes.
Conclusions:
- Proteomics is instrumental in characterizing PTMs in P. aeruginosa.
- Understanding PTMs provides insights into bacterial adaptation and virulence.
- Modified proteins and enzymes represent promising targets for novel therapeutics against P. aeruginosa infections.
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