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Published on: September 25, 2014
[Rapid peroxidase test for demonstrating the bovine pestivirus in cell cultures]
Veterinarno-Meditsinski Nauki
|January 1, 1986
Abstract:
Different variants were tested of the immunoperoxidase method for the demonstration of the mucous disease-virus diarrhoea agent in infected cells with the use of a specific conjugated antiserum. It was found that the virus could be specifically demonstrated in infected cell cultures. The test proved rapid and readily applicable.
Insights
The immunoperoxidase method effectively detects the mucous disease-virus diarrhoea agent in infected cells. This rapid and applicable test specifically identifies the virus in cell cultures.
Area of Science:
- Veterinary Virology
- Immunohistochemistry
- Cell Biology
Context:
- Mucous disease-virus diarrhoea (MDV-D) is a significant pathogen in animal health.
- Accurate and rapid diagnostic methods are crucial for disease control.
- The immunoperoxidase method offers potential for sensitive antigen detection.
Purpose:
- To evaluate different variants of the immunoperoxidase method for detecting the MDV-D agent.
- To assess the specificity and applicability of the method in infected cell cultures.
Summary:
- Various immunoperoxidase method protocols were tested for demonstrating the MDV-D agent.
- The virus was specifically identified in infected cell cultures using a conjugated antiserum.
- The optimized method proved to be rapid and readily applicable for diagnostic purposes.
Impact:
- Provides a validated, rapid diagnostic tool for MDV-D.
- Facilitates timely disease identification and management in affected animal populations.
- Contributes to improved disease surveillance and control strategies.
