Mouse incising central pattern generator: Characteristics and modulation by pain

Vertebrate incising and chewing are controlled by a set of neurons comprising the central pattern generator (CPG) for mastication. Mandibular positioning and force generation to perform these tasks is complex and requires coordination of multiple jaw opening and closing muscle compartments located in muscles on both sides of the jaw. The purpose of this study was to determine the characteristics of the CPG by recording mouse incising forces in the home cage environment to evaluate changes in force characteristics with incising frequency and force direction. A second purpose was to evaluate the effects of jaw closing muscle pain on CPG output parameters.

Digitized incising forces were recorded for approximately 24 h using a 3-dimensional force transducer attached to solid food chow. Male and female CD-1 mice were evaluated during their last (fourth) baseline assessment and seven days after a second acidic saline injection into the left masseter muscle when maximum pain was experienced. Incising force resultants were calculated from the three axes data and force parameters were assessed including inter-peak intervals (IPI), peak amplitude, load time and unload time. Multiple regression analyses were conducted to identify incising episodes that had parameters of force that were significantly correlated (p < 0.001). These incising episodes were considered to represent the output of the CPG with a steady state of incoming sensory afferent inputs. Incising parameters were evaluated for each of the discrete incising frequencies (4.6, 5.3, 6.2, 7.6 Hz) and the predominant force directions: jaw closing (-Z), jaw retrusion (+X) and jaw protrusion (-X).

A significant correlation between incising frequency (IPI) and the load time was observed. A significant decrease in peak amplitude was observed with higher incising frequency while the load rate significantly increased. The force peak amplitude and load rates were found to be statistically different when the force direction was considered, with smaller peak amplitudes and smaller load rates found in the jaw closing direction. The effect of pain on incising was to reduce the peak amplitude and load rate of incising compared to the baseline condition at lower incising frequencies.

Like the central pattern generator for locomotion, the CPG for incising controls rhythmicity, peak amplitude and force load duration/rate. However, unlike the CPG for locomotion, the amplitude of incising force decreases as the frequency increases. During incising, load rate increases with faster rhythm and is consistent with the recruitment of larger motor units. Muscle pain reduced the excitatory drive of the CPG on motoneurons and provides further support of the Pain Adaptation Model.