Theoretical Analysis for Bending of Single-Stranded DNA Adsorption on Microcantilever Sensors
Zou-Qing Tan1, Yang-Chun Chen2, Neng-Hui Zhang3,4
1School of Mechanical Engineering, Changzhou University, Changzhou 213164, China. zqtan@cczu.edu.cn.
Abstract:
An energy-based model is presented to establish the bending deformation of microcantilever beams induced by single-stranded DNA (ssDNA) adsorption. The total free energy of the DNA-microcantilever sensor was obtained by considering the excluded-volume energy and the polymer stretching energy of DNA chains from mean-field theory, and the mechanical energy of three non-biological layers. The radius of curvature and deflection of the cantilever were determined through the minimum principle of energy. The efficiency of the present model was confirmed through comparison with experimental data. The effects of length, grafting density, salt concentration, thickness, and elastic modulus of substrate on tip deflections are also discussed in this paper. These factors can significantly affect the deflections of the biosensor. This work demonstrates that it is useful to develop a theoretical model for the label-free nanomechanical detection technique.
Related Concept Videos
Single-Strand DNA Binding Proteins
Lagging Strand Synthesis
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
Fixing Double-strand Breaks
Bending of Curved Members - Strain Analysis
The important part of bending analysis for such a member...
DNA Helicases
DNA Topoisomerases
Types and Mechanism of action
Topoisomerases are divided into two main types. ...


