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Mechanically Enhancing Planar Lipid Bilayers with a Minimal Actin Cortex.

Daniel L Burden1, Daniel Kim1, Wayland Cheng1

  • 1Chemistry Department , Wheaton College , Wheaton , Illinois 60187 , United States.

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Summary

We developed a minimal actin cortex (MAC) to mechanically support fragile black lipid membranes (BLMs). This actin-supported BLM composite enhances stability and is ideal for nanopore sensing applications.

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Area of Science:

  • Biophysics
  • Materials Science
  • Cell Biology

Background:

  • Cells utilize a cytoskeleton for mechanical stability.
  • Fragile black lipid membranes (BLMs) are crucial for nanopore sensing but require mechanical support.
  • Existing methods for supporting BLMs can be complex or limit their functionality.

Purpose of the Study:

  • To create a mechanically robust, yet functional, biomembrane composite for advanced sensor applications.
  • To investigate the structural and electrical properties of actin-supported lipid bilayers.
  • To assess the potential of this composite for ion-channel and nanopore sensing.

Main Methods:

  • Formation of a minimal actin cortex (MAC) by anchoring actin filaments to BLMs using a biotin-streptavidin-biotin linkage.
  • Characterization using optical microscopy, electrophysiology, and mechanical stress tests.
  • Measurement of elastic modulus and electrical resistance of the composite structure.

Main Results:

  • The minimal actin cortex (MAC)-bilayer composite demonstrated significantly enhanced mechanical stability (>100× increase in elastic modulus).
  • The composite maintained high electrical resistance and lateral fluidity of the BLM.
  • The structure remained stable for hours to days, with actin filaments intact for months, allowing molecular access and nanopore insertion.

Conclusions:

  • A minimal actin cortex provides robust mechanical support to black lipid membranes, creating a stable and functional composite.
  • This actin-supported BLM system is highly promising for developing advanced ion-channel and protein nanopore sensing technologies.
  • The method offers a versatile platform for enhancing biomembrane stability without compromising essential electrical and fluidic properties.