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Updated: Jul 12, 2026
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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Published on: July 9, 2008
'NOTCHing up' the In Vitro Production of Dendritic Cells
Martin Guilliams1, Charlotte L Scott2
1Laboratory of Myeloid Cell Ontogeny and Functional Specialization, VIB-UGent Center for Inflammation Research, Ghent, Belgium; Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium.
Trends in Immunology
|August 29, 2018
Abstract:
Two studies show that adding NOTCH ligands to Flt3L cultures increases the yield of cDC1s from mouse and human progenitors. Moreover, these NOTCH-DCs more closely resemble in vivo DCs transcriptionally and functionally. This will be extremely valuable for studying DCs in vitro and for further developing autologous DC-based antitumor vaccinations.
