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Updated: Feb 6, 2026
08:56
Simultaneous Imaging and Flow-Cytometry-based Detection of Multiple Fluorescent Senescence Markers in Therapy-Induced Senescent Cancer Cells
Published on: July 12, 2022
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Senescence markers: Predictive for response to checkpoint inhibitors
Alvaro Moreira1, Stefanie Gross1, Michael Constantin Kirchberger1
1Department of Dermatology, University Hospital Erlangen, Friedrich-Alexander-University Erlangen-Nürnberg (FAU), Erlangen, Germany.
International Journal of Cancer
|August 29, 2018
Summary
Immunosenescence, or immune system aging, affects how well immune checkpoint inhibitors work for melanoma. Specific T cell markers can predict patient response to this cancer therapy.
Area of Science:
- Immunology
- Oncology
- Translational Medicine
Background:
- Age-related immune system changes, termed immunosenescence, may influence the effectiveness of immune checkpoint inhibitors (ICIs).
- Previous research suggests potential impacts of immunosenescence on ICI efficacy in leukemia and non-small cell lung cancer.
Purpose of the Study:
- To determine if senescence markers can predict treatment response in melanoma patients receiving ICIs.
- To identify specific immunosenescence phenotypes associated with ICI therapy outcomes.
Main Methods:
- Analysis of peripheral blood from newly diagnosed, untreated metastatic melanoma patients.
- Flow cytometry was used to correlate senescence marker frequency with clinical response (RECIST) after 12 weeks of ICI treatment.
Main Results:
- Loss of T cell surface markers CD27 and CD28 was linked to resistance to ICI therapy.
- Expression of T cell markers Tim-3 and CD57 also correlated with resistance to ICI blockade.
- These T cell phenotypes are potential predictive biomarkers for ICI therapy response.
Conclusions:
- Immunosenescence appears to significantly impact the response of melanoma patients to ICI therapy.
- Lymphocyte phenotyping for senescence markers could serve as a predictive tool for ICI response.
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