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Updated: Feb 6, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
Genome Editing of MSCs as a Platform for Cell Therapy
Krissanapong Manotham1, Supreecha Chattong2,3
1Molecular and Cellular Biology Unit, Department of Medicine, Lerdsin General Hospital, Bangkok, Thailand. kmanotham@hotmail.com.
Abstract:
The rapid growing of genome editing technology leads to an optimistic expectation for the treatment of many incurable diseases. The core of genome editing relies on DNA-repairing processes which occur independently in each cell, thus generating a mix of genetic variation cells. Here, we describe the protocol of using mesenchymal stem cells as a platform to generate high purity of ZFN-edited patients' cell clones which may be useful in conjunction with therapeutic cell conversion and reprograming.
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