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Updated: Feb 6, 2026

Primer-Free Aptamer Selection Using A Random DNA Library
Published on: July 26, 2010
Selection and Characterization of DNA Aptamers Against FokI Nuclease Domain
Maui Nishio1, Ayana Yamagishi2, Kaori Tsukakoshi1
1Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, Tokyo, Japan.
Abstract:
Genome editing with site-specific nucleases (SSNs) may be effective for gene therapy, as SSNs can modify target genes. However, the main limitation of genome editing for clinical use is off-target effects by excess amounts of SSNs within cells. Therefore, a controlled delivery system for SSNs is necessary. Previously we have reported on a zinc finger nuclease (ZFN) delivery system, which combined DNA aptamers against FokI nuclease domain (FokI) and nanoneedles. Here, we describe how DNA aptamers against FokI were selected and characterized for genome editing applications.
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