Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Relaxation experiments using bath-applied suberyldicholine.

P R Adams

    The Journal of Physiology
    |June 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hebbian learning from higher-order correlations requires crosstalk minimization.

    Biological cybernetics·2014
    Same author

    Uptake of nitrate, ammonium and glycine by plants of Tasmanian wet eucalypt forests.

    Tree physiology·2007
    Same author

    Optimal sample preparation conditions for the determination of uranium in biological samples by kinetic phosphorescence analysis (KPA).

    Journal of pharmaceutical and biomedical analysis·2000
    Same author

    Traumatic aortic transections: eight-year experience with the "clamp-sew" technique.

    The Annals of thoracic surgery·1997
    Same author

    Visualization of calcium influx through channels that shape the burst and tonic firing modes of thalamic relay cells.

    Journal of neurophysiology·1997
    Same author

    Extracellular amylase activities of Rhizomucor pusillus and Humicola lanuginosa at initial stages of growth.

    Mycopathologia·1994
    Same journal

    Systems modelling of mitochondrial dynamics in different exercise regimes.

    The Journal of physiology·2026
    Same journal

    Kinematic-calcium loops unravel impaired excitation-contraction coupling in MELAS-affected cardioids.

    The Journal of physiology·2026
    Same journal

    hERG1 channels and potential therapeutics for long QT syndrome.

    The Journal of physiology·2026
    Same journal

    A high-fat, high-sucrose diet exacerbates muscle and metabolic pathology and undermines glucocorticoid efficacy in dystrophin-deficient mice.

    The Journal of physiology·2026
    Same journal

    Ca<sub>V</sub>1.2 dynamics in native male arterial myocytes.

    The Journal of physiology·2026
    Same journal

    Glutamatergic modulation of transcranial direct current stimulation over the premotor cortex combined with peripheral nerve stimulation promotes observation-execution-related cortical excitability and motor performance.

    The Journal of physiology·2026
    See all related articles

    Researchers studied how membrane potential affects muscle fiber conductance using suberyldicholine. They found that conductance changes exponentially with voltage, suggesting a new model involving binding and isomerization steps.

    Area of Science:

    • Neuroscience
    • Muscle Physiology
    • Biophysics

    Background:

    • Nicotinic acetylcholine receptors (nAChRs) mediate neuromuscular transmission.
    • Understanding the kinetics of nAChR activation is crucial for neuromuscular function.

    Purpose of the Study:

    • To investigate the dynamic response of end-plate conductance to voltage changes.
    • To elucidate the mechanism of suberyldicholine-induced conductance changes in frog muscle fibers.

    Main Methods:

    • Voltage-clamped frog muscle fibers were utilized.
    • Bath application of suberyldicholine was employed to induce conductance changes.
    • Step changes in membrane potential were applied to assess dynamic responses.

    Main Results:

    Related Experiment Videos

    • Suberyldicholine-induced conductance exhibited exponential relaxation towards new equilibrium levels after voltage steps.
    • At low suberyldicholine concentrations, relaxation time constants were concentration-independent and mirrored voltage dependence of equilibrium conductance.
    • High suberyldicholine concentrations resulted in less voltage-sensitive conductance compared to low concentrations.
    • Bungarotoxin pretreatment reduced response amplitude but did not alter the fundamental kinetics.

    Conclusions:

    • A novel kinetic model is proposed, involving rate-limiting ligand binding followed by voltage-dependent isomerization.
    • This model explains both noise and relaxation data, providing insights into nAChR gating mechanisms.