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Updated: Feb 6, 2026

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase
Published on: September 22, 2012
Histone Deacetylase Inhibitor Scriptaid Alleviated Neurological Dysfunction after Experimental Intracerebral
Heng Yang1, Wei Ni1, Hanqiang Jiang1
1Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, China.
Objectives:
To investigate the role of Scriptaid in reducing brain injury after intracerebral hemorrhage (ICH) in mice.
Methods:
An ICH model was constructed by injecting autologous blood into the right basal ganglia in mice. The animals were administered 3.5 mg/kg of Scriptaid intraperitoneally after ICH. The hematoma volume and hemoglobin level were measured to examine hematoma resolution. A behavior test and brain edema and white matter injury examinations indicated brain injury after ICH.
Results:
Scriptaid treatment promoted hematoma resolution and reduced the hematoma volume 7 d after ICH compared with the vehicle group (P < 0.05). Scriptaid treatment also alleviated the brain water content in the ipsilateral basal ganglia (P < 0.05) and cortex (P < 0.01) 3 d after ICH. In addition, Scriptaid improved neurological function recovery and alleviated white matter injury 35 d after ICH.
Conclusions:
Scriptaid can protect against brain injury after ICH and may be considered a new medical therapy method for ICH.
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