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A novel protocol for bacterial ghosts' preparation using tween 80.

Sameh Rabea1,2, Mounir M Salem-Bekhit2,3, Fars K Alanazi2

  • 1Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo 11652, Egypt.

Saudi Pharmaceutical Journal : SPJ : the Official Publication of the Saudi Pharmaceutical Society
|September 1, 2018
PubMed
Summary

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This summary is machine-generated.

This study introduces a novel, cost-effective method for producing bacterial ghosts (BGs) using tween 80 and lactic acid. The new protocol yields high-quality BGs from Salmonella typhimurium, confirmed by SEM and viability tests.

Area of Science:

  • Microbiology
  • Biotechnology

Background:

  • Bacterial ghosts (BGs) are non-living bacterial cells with potential applications in biotechnology and medicine.
  • Existing methods for BG preparation include genetic and chemical approaches, each with limitations.

Purpose of the Study:

  • To develop a new, simple, economic, and feasible protocol for producing high-quality bacterial ghosts.
  • To utilize tween 80 and a sudden pH reduction for BG preparation.

Main Methods:

  • Salmonella enterica serovar typhimurium ATCC 13311 cells were incubated in 7% tween 80 for 24 hours at 37°C.
  • The pH was then reduced to 3.6 using lactic acid for one hour.
  • Bacterial pellets were collected via centrifugation, washed, and analyzed using SEM, subculturing, and light microscopy.
Keywords:
Bacterial ghostsBiotechnology platformDrug delivery systemS. TyphimuriumTween 80

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Main Results:

  • Scanning electron microscopy confirmed the production of high-quality BGs with intact shells and intramembranous tunnels.
  • Subculturing confirmed the absence of viable cells, and protein/DNA release indicated ghost formation.
  • Gram staining verified cell integrity, demonstrating the effectiveness of the protocol.

Conclusions:

  • The developed protocol offers a simple, economical, and feasible method for bacterial ghost preparation.
  • This new approach yields high-quality bacterial ghosts suitable for further applications.
  • The method is effective for Salmonella typhimurium and potentially other bacterial species.