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Related Experiment Video

Updated: Feb 5, 2026

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

Published on: July 6, 2019

Synchronization-free all-solid-state laser system for stimulated Raman scattering microscopy.

Tobias Steinle¹, Vikas Kumar², Moritz Floess¹

¹4th Physics Institute and Research Center SCoPE, University of Stuttgart, D-70569, Stuttgart, Germany.

Light, Science & Applications

|September 1, 2018

Summary

This summary is machine-generated.

We developed a simple and stable stimulated Raman scattering (SRS) microscopy system. This new system achieves high chemical contrast imaging of plant tissues without complex detection setups.

Keywords:

label-free imaging optical parametric amplifier spectral compression stimulated Raman scattering

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Implementation of a Nonlinear Microscope Based on Stimulated Raman Scattering

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Area of Science:

Optics and Photonics
Microscopy
Biomedical Imaging

Background:

Stimulated Raman scattering (SRS) microscopy offers label-free chemical contrast imaging.
Existing SRS systems can be complex and require sophisticated detection methods.
High stability and simplicity are crucial for widespread adoption of SRS microscopy.

Purpose of the Study:

To introduce a highly stable and simple SRS microscopy system.
To demonstrate the system's capability for high-contrast chemical imaging.
To evaluate the system's performance for biological sample analysis.

Main Methods:

Utilized a Yb:KGW bulk oscillator and an optical parametric amplifier for tunable pump pulses.
Employed a continuous-wave (cw) tunable external cavity diode laser for seeding.
Generated narrowband picosecond pump and Stokes pulses for SRS excitation.

Main Results:

Achieved SRS microscopy with a 30-μs pixel dwell time.
Demonstrated high chemical contrast and signal-to-noise ratio (>45).
Showcased the system's stability and simplicity, eliminating the need for balanced detection.

Conclusions:

The developed SRS microscopy system is simple, stable, and effective.
It provides excellent chemical contrast for imaging biological samples like plant tissues.
The system's favorable noise properties and low spectral drift enable robust imaging.