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Related Concept Videos

Transcription Factors02:16

Transcription Factors

82.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Transcription Elongation Factors02:35

Transcription Elongation Factors

14.0K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
14.0K
Transcription Elongation Factors02:35

Transcription Elongation Factors

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4.8K
General Transcription Factors01:30

General Transcription Factors

7.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.1K
Transcription01:10

Transcription

156.7K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
156.7K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K

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Related Experiment Video

Updated: Feb 5, 2026

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

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Programmable T7-based synthetic transcription factors.

Brendan J Hussey1,2,3, David R McMillen1,2,3

  • 1Chemical and Physical Sciences, University of Toronto Mississauga, Mississauga, Ontario L5L 1C6, Canada.

Nucleic Acids Research
|September 1, 2018
PubMed
Summary

Scientists developed a new synthetic transcription activator system for bacteria. This programmable system uses T7 RNA polymerase for high-activity gene control, offering orthogonality and modularity in Escherichia coli.

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Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Microbiology

Background:

  • Synthetic transcription factors are crucial for precise gene regulation.
  • Existing bacterial systems lack high activity, orthogonality, and programmability.
  • Bacteriophage T7 RNA polymerase offers high transcription rates and modularity.

Purpose of the Study:

  • To engineer a novel, high-activity, programmable transcription activator system for bacteria.
  • To leverage T7 RNA polymerase for synthetic gene expression control in Escherichia coli.
  • To establish an orthogonal and modular synthetic transcription factor platform.

Main Methods:

  • Recruitment of T7 RNA polymerase to specific DNA sites using engineered DNA-binding proteins.
  • Utilizing direct or protein-protein interaction-mediated bridging for polymerase recruitment.
  • Development of multiple orthogonal synthetic transcription factor variants.

Main Results:

  • Successful creation of a modular and programmable transcription activation system in Escherichia coli.
  • Demonstration of high transcriptional activation rates using the T7 RNA polymerase-based system.
  • Establishment of an exogenous, programmable activator system, novel for bacterial applications.

Conclusions:

  • The developed system provides a powerful tool for synthetic biology applications in bacteria.
  • This platform enables precise and strong control over gene expression.
  • It represents the first exogenous, programmable activator system for bacteria, paving the way for advanced synthetic biology designs.