Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

37.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
37.3K
Immunoprecipitation01:20

Immunoprecipitation

5.1K
Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A framework to enhance the signal-to-noise ratio for quantitative fluorescence microscopy.

PloS one·2025
Same author

Malarial Antibody Detection with an Engineered Yeast Agglutination Assay.

ACS synthetic biology·2022
Same author

E.coli Nissle increases transcription of flagella assembly and formate hydrogenlyase genes in response to colitis.

Gut microbes·2021
Same author

Improving the design of an oxidative stress sensing biosensor in yeast.

FEMS yeast research·2021
Same author

Design and Experimental Validation of Small Activating RNAs Targeting an Exogenous Promoter in Human Cells.

ACS synthetic biology·2016
Same author

Design principles for the analysis and construction of robustly homeostatic biological networks.

Journal of theoretical biology·2016

Related Experiment Video

Updated: May 15, 2025

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
07:56

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

18.1K

An inhibitor-free, versatile, fast, and cheap precipitation-based DNA purification method.

Zhe F Tang1, David R McMillen1

  • 1Department of Chemical and Physical Sciences, University of Toronto, Mississauga, Ontario, Canada.

Plos One
|April 8, 2025
PubMed
Summary

A new nucleic acid purification method offers a cheaper, faster alternative to silica columns, overcoming enzyme inhibition and improving DNA transformation efficiency. This single-step process enhances synthetic biology workflows.

More Related Videos

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

11.6K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

27.7K

Related Experiment Videos

Last Updated: May 15, 2025

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method
07:56

Filtration Isolation of Nucleic Acids: A Simple and Rapid DNA Extraction Method

Published on: August 6, 2016

18.1K
Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

11.6K
Electroeluting DNA Fragments
06:13

Electroeluting DNA Fragments

Published on: September 5, 2010

27.7K

Area of Science:

  • Molecular Biology
  • Synthetic Biology
  • Biochemistry

Background:

  • Nucleic acid purification is essential for molecular biology and synthetic biology.
  • Commercial silica columns can elute an unidentified substance inhibiting enzymatic reactions.
  • Existing DNA precipitation methods are multi-step and less efficient.

Purpose of the Study:

  • To develop a novel, single-step nucleic acid purification method.
  • To address the limitations of current purification techniques, including enzyme inhibition and cost.
  • To investigate the impact of DNA structure on transformation efficiency.

Main Methods:

  • Developed a purification approach using chaotropic salts and alcohol/polyethylene glycol for simultaneous protein removal and DNA precipitation.
  • Compared the novel method's efficiency, cost, and DNA fragment size compatibility with silica column purification.
  • Investigated the effect of DNA concentration and form (linear vs. circular) on bacterial transformation.

Main Results:

  • The novel method achieves protein removal and DNA precipitation in a single step, outperforming multi-step protocols.
  • This technique is cheaper than silica columns, avoids DNA size restrictions, and eliminates the need for gel extraction of small fragments.
  • Discovered that linear DNA from Gibson Assembly transforms poorly; reducing its concentration significantly increases circularization and improves transformation.

Conclusions:

  • The developed purification method is a cost-effective, versatile alternative to silica columns for molecular biology applications.
  • The study reveals critical insights into DNA structure's impact on transformation efficiency in E. coli.
  • This purification technique facilitates improved synthetic biology workflows and DNA assembly analysis.