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Updated: Feb 5, 2026
09:46
Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
Published on: December 27, 2017
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Imaging Platelets and Megakaryocytes by High-Resolution Laser Fluorescence Microscopy
Fred G Pluthero1, Walter H A Kahr2,3,4
1Cell Biology Program, Research Institute, Hospital for Sick Children, Toronto, Ontario, Canada.
Methods in Molecular Biology (Clifton, N.J.)
|September 2, 2018
Summary
This study details methods for high-resolution immunofluorescence microscopy of platelets and megakaryocytes. These protocols enable rapid, detailed imaging of these critical blood cells and their precursors.
Area of Science:
- Hematology
- Cell Biology
- Microscopy
Background:
- Microscopy is essential for studying platelets and megakaryocytes.
- Existing methods may lack speed or resolution for detailed cellular analysis.
Purpose of the Study:
- To describe rapid, high-resolution immunofluorescence microscopy methods for platelets and megakaryocytes.
- To provide validated protocols for cell preparation, staining, imaging, and analysis.
Main Methods:
- Cell isolation and preparation for fluorescence staining.
- Immunofluorescence staining using validated antibodies.
- High-resolution imaging with spinning-disc confocal and structured illumination microscopy.
- Image processing and presentation techniques.
Main Results:
- Successful rapid acquisition of high-resolution images of fixed platelets, megakaryocytes, and megakaryocytic cells.
- Validated list of primary antibodies for specific protein and subcellular structure staining.
Conclusions:
- The described immunofluorescence microscopy methods facilitate rapid, high-resolution imaging of platelets and megakaryocytes.
- These protocols and validated reagents aid in detailed cellular studies.
Keywords:
Cell imagingConfocalImmunofluorescenceLaserMegakaryocyteMicroscopyPlateletSpinning-discStructured illuminationMore Related Videos
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