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Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition
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Negative Staining Electron Microscopy of a Highly Flexible Sec1/Munc18 Protein Complex Stabilized by Glutaraldehyde

Richard J Y Liu1, Walter H A Kahr2,3,4

  • 1Departments of Paediatrics and Biochemistry, University of Toronto, Toronto, ON, Canada.

Methods in Molecular Biology (Clifton, N.J.)
|January 13, 2025
PubMed

Keywords:
AvidinCrosslinkingElectron microscopyNegative stainingProtein complexSec1/Munc18Structure determination

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