Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

What is Metabolism?00:52

What is Metabolism?

131.9K
Overview
131.9K
C4 Pathway and CAM01:27

C4 Pathway and CAM

49.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.2K
Other Glycolytic Pathways01:24

Other Glycolytic Pathways

917
The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
917
Carbohydrate Metabolism01:36

Carbohydrate Metabolism

14.3K
Carbohydrates are polymers composed of molecules containing atoms of carbon, hydrogen and oxygen. One gram of carbohydrate can provide four kilo-calories of energy, which makes it the most efficient instant energy source.
Starch accounts for approximately 60% of the carbohydrates consumed by humans. Since amylase enzymes cannot function in the stomach's acidic environment, starch can only be digested in the mouth and small intestine. Simple sugars are found naturally in milk and fruits in...
14.3K
Auditory Pathway01:15

Auditory Pathway

7.4K
Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...
7.4K
ER Retrieval Pathway01:45

ER Retrieval Pathway

4.8K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
4.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Moonlighting in metabolism: bifunctional enzymes control nitrogen metabolism in <i>Bacillus subtilis</i>.

Microbiology and molecular biology reviews : MMBR·2026
Same author

Real-world analysis of outcomes of patients undergoing coronary intravascular lithotripsy versus atherectomy for calcified coronary artery disease: findings from the TriNetX database.

The Journal of invasive cardiology·2026
Same author

AI-BLADE toolbox: AI-powered BLADdEr multiparametric MRI analysis for clinical application.

BJR artificial intelligence·2026
Same author

The evolution of a Na<sup>+</sup>-sensitive <i>Vibrio cholerae</i> mutant unmasks the moonlighting aminopeptidase PepA as a regulator of <i>nhaB</i> Na<sup>+</sup>/H<sup>+</sup> antiporter gene expression.

bioRxiv : the preprint server for biology·2026
Same author

Advanced Urologic Cancer Consensus Conference (AUC3) 2025: Expert consensus on the management of renal cell and urinary tract cancers.

CA: a cancer journal for clinicians·2025
Same author

First Reported Case of Infection Involving a Leadless Atrial Pacemaker.

JACC. Case reports·2025
Same journal

Engineering environmental bacteria for whole-cell PET hydrolysis and assimilation.

Trends in biotechnology·2026
Same journal

Acoustic cavitation-enhanced lymphatic trafficking of inhaled bacterial-sourced biohybrid vaccines for antitumor immunity.

Trends in biotechnology·2026
Same journal

Make uphill thermodynamics downhill in pathway design.

Trends in biotechnology·2026
Same journal

Engineering a capture-bioremediate-release microbial biofilm for simultaneous bioremediation of microplastics and adsorbed heavy metals.

Trends in biotechnology·2026
Same journal

Engineered bacterial biofilms for biotechnological applications.

Trends in biotechnology·2026
Same journal

Multiscale and programmable engineering of edible mushroom mycelium-based materials.

Trends in biotechnology·2026
See all related articles

Related Experiment Video

Updated: Feb 5, 2026

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
05:01

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Published on: July 1, 2020

3.8K

Harnessing Underground Metabolism for Pathway Development.

Jonathan Rosenberg1, Fabian M Commichau1

  • 1Department of General Microbiology, University of Göttingen, Grisebachstrasse 8, 37077 Göttingen, Germany.

Trends in Biotechnology
|September 3, 2018
PubMed
Summary
This summary is machine-generated.

This study proposes a novel metabolic engineering strategy for enhanced fermentative production of valuable compounds. By rewiring hybrid pathways, we can overcome metabolic hurdles and leverage the host

Keywords:
adaptive laboratory evolutionconvergent evolutionenzyme promiscuitymetabolic engineeringpathway evolutionsuppressor mutation

More Related Videos

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.0K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.5K

Related Experiment Videos

Last Updated: Feb 5, 2026

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
05:01

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Published on: July 1, 2020

3.8K
Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
07:26

Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids

Published on: January 26, 2012

25.0K
Metabolic Profile Analysis of Zebrafish Embryos
05:41

Metabolic Profile Analysis of Zebrafish Embryos

Published on: January 14, 2013

20.5K

Area of Science:

  • Metabolic Engineering
  • Synthetic Biology
  • Biotechnology

Background:

  • Fermentative production offers sustainable alternatives to chemical synthesis but faces challenges from host-pathway interactions.
  • Understanding and mitigating these metabolic conflicts is crucial for efficient bioproduction.
  • The concept of 'underground metabolism' presents an untapped resource for pathway engineering.

Purpose of the Study:

  • To propose a novel approach for engineering hybrid metabolic pathways.
  • To address detrimental interactions between heterologous pathways and host metabolism.
  • To explore the potential of harnessing intrinsic cellular capacity for biotechnological applications.

Main Methods:

  • Metabolic rewiring of reversely engineered hybrid pathways.
  • Leveraging knowledge of convergently evolved pathways.
  • Recruiting the host's 'underground metabolism'.

Main Results:

  • The proposed approach offers a universally applicable strategy.
  • It presents a technologically simple method for metabolic engineering.
  • It aims to circumvent common problems in heterologous pathway expression.

Conclusions:

  • Metabolic rewiring of hybrid pathways is a promising strategy for improved bioproduction.
  • Harnessing intrinsic cellular metabolism can advance food and feed technologies.
  • This approach offers a viable alternative to traditional chemical synthesis.