Related Experiment Video
Updated: Feb 5, 2026

Author Spotlight: Quantification of Complex Lipidomic Samples Using Stable Isotope Labeling
Published on: August 23, 2024
Identification of naringin metabolites mediated by human intestinal microbes with stable isotope-labeling method and
Taobin Chen1, Weiwei Su1, Zenghao Yan2
1Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China; Shenzhen Research Institute of Sun Yat-sen University, Shenzhen 518057, China.
Abstract:
Widely presented in medicinal plants, naringin is one of the major flavanones with various pharmaceutical bioactivities. After oral administration, naringin predominantly undergoes metabolisms mediated by liver cytochrome P450 and gut microbes, while its human microbes-mediated metabolic profiling is still largely obscure due to the endogenous interferences, which makes it extremely difficult to analyze metabolites precisely. In this study, we aim of systematically investigating the biotransformation of naringin mediated by human intestinal microbes through applying stable isotope-labeling method. [2',3',5',6'-D4]naringin was synthesized and incubated anaerobically with human gut microbes. A total of 13 microbial metabolites were detected and identified by UFLC-Q-TOF-MS/MS, among which 5 were reported for the first time. Furthermore, the proposed metabolic pathway revealed that naringin went through extensive phase I metabolism in human intestinal microbes. Of note, diverse metabolic profiles of naringin among human participants were obtained, which could be attributed to the distinct gut microbiota compositions of individuals.
More Related Videos
Related Concept Videos
Isotopes
An element's atomic mass, or weight,...
Elements: Chemical Symbols and Isotopes
Some symbols are derived from the common English name of the element; others are abbreviations of the name in another language — Latin, Greek or German. For example, the symbol for aluminum (common name)...
Methods of Classification and Identification
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...
Isotopes and Radioisotopes
An isotope containing...
Mass Spectrometry: Isotope Effect

