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Label free DNA-based optical biosensor as a potential system for wine authenticity.

Sara Barrias1, José R Fernandes2, José Eduardo Eiras-Dias3

  • 1University of Trás-os-Montes and Alto Douro, 5001-801 Vila Real, Portugal; University of Lisboa, Faculty of Sciences, BioISI - Biosystems & Integrative Sciences Institute Campo Grande, C8 bdg, 1749-016 Lisboa, Portugal.

Food Chemistry
|September 4, 2018
PubMed
Summary

This study demonstrates a DNA biosensor

Keywords:
AuthenticityDNA-based biosensorVitis vinifera L.Wine

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Area of Science:

  • Food Science and Technology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Vitis vinifera L. diversity enables varied wine production.
  • Food composition analysis platforms are emerging.
  • DNA biosensors offer applications in food safety and authenticity.

Purpose of the Study:

  • To evaluate a DNA-based optical biosensor's detection capability.
  • To test the biosensor's performance with diverse Vitis vinifera matrices (leaf, must, wine).

Main Methods:

  • Genomic DNA extraction from leaf, must, and wine of three V. vinifera varieties.
  • Testing extracted DNA on a developed long-period grating (LPG) DNA-based biosensor.

Main Results:

  • The biosensor successfully distinguished between V. vinifera varieties.
  • Effective detection was achieved even with DNA from complex matrices.
  • The biosensor demonstrated high specificity and sensitivity.

Conclusions:

  • The DNA-based optical biosensor shows significant potential for wine authenticity analysis.
  • This technology can differentiate grape varieties from various sample types.
  • Further development could enhance its application in food traceability.