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Fluorescence strategy for sensitive detection of adenosine triphosphate in terms of evaluating meat freshness
Zhenning Liu1, Yinghui Zhong1, Yue Hu2
1College of Food Science and Engineering, Jilin University, Changchun 130062, China; Key Laboratory of Bionic Engineering (Ministry of Education, China), College of Biological and Agricultural Engineering, Jilin University, Changchun 130022, China.
Abstract:
A novel simple, sensitive and reliable sensor based on S1 nuclease, FAM-labeled ssDNA (DNA-F) and graphene oxide (GO) was developed for detecting adenosine triphosphate (ATP) and evaluating the freshness of meat (beef) samples. With S1 nuclease as the cleaver of DNA-F and ATP as the inhibitor of S1 nuclease, the fluorescence of DNA-F could be obviously quenched by GO, which exhibits the fluorescence of system gradually decrease as the increasing ATP concentration. Under the optimal conditions, a linear correlation between the fluorescence and the ATP concentration from 20 μM to 3500 μM is obtained with a detection limit of 3.2 μM. Furthermore, the proposed ATP detection method was applied to the ATP detection in microorganisms in meat samples, which acquired the satisfying results, respectively.
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