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Covalently Assembled Dipeptide Nanoparticles with Adjustable Fluorescence Emission for Multicolor Bioimaging
Shukun Li1, Yamei Liu1, Ruirui Xing1
1State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 North Second Street, Zhongguancun, Beijing, 100190, P.R. China.
Chembiochem : a European Journal of Chemical Biology
|September 4, 2018
Summary
Researchers developed stable, fluorescent nanoparticles using covalently crosslinked peptide self-assembly. This method allows for tunable emission, enhancing multicolor bioimaging applications with robust physiological stability.
Area of Science:
- Biomaterials Science
- Nanotechnology
- Bioimaging
Background:
- Peptide self-assembly is attractive for creating fluorescent nanoagents for bioimaging.
- Noncovalent interactions in peptide self-assembly are vulnerable to physiological conditions, causing nanoagent disassembly and dysfunction.
Purpose of the Study:
- To introduce a strategy for fabricating stable peptide-based nanoparticles with adjustable emission using covalent crosslinking.
- To overcome the limitations of noncovalent peptide self-assembly in physiological environments.
Main Methods:
- Utilized cationic diphenylalanine peptide (H-Phe-Phe-NH2·HCl) self-assembly.
- Employed glutaraldehyde as a crosslinker, forming fluorescent Schiff base products.
- Controlled covalent reaction time to tune emission wavelength.
Main Results:
- Successfully fabricated stable, peptide-based nanoparticles.
- Demonstrated tunable emission wavelengths by adjusting covalent reaction time.
- Confirmed nanoparticle stability after intracellular uptake, enabling sustainable multicolor fluorescent imaging.
Conclusions:
- The covalent crosslinking strategy enhances the physiological stability of peptide-based fluorescent nanoparticles.
- This approach offers a promising route for engineering functional fluorescent nanoparticles with adjustable emission for advanced bioimaging.
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