Jove
Visualize
Contact Us

Related Concept Videos

Group Design02:01

Group Design

10.6K
The most basic experimental design involves two groups: the experimental group and the control group. The two groups are designed to be the same except for one difference— experimental manipulation. The experimental group gets the experimental manipulation—that is, the treatment or variable being tested—and the control group does not. Since experimental manipulation is the only difference between the experimental and control groups, we can be sure that any differences between...
10.6K
Factorial Design02:01

Factorial Design

13.8K
Factorial Analysis is an experimental design that applies Analysis of Variance (ANOVA) statistical procedures to examine a change in a dependent variable due to more than one independent variable, also known as factors. Changes in worker productivity can be reasoned, for example, to be influenced by salary and other conditions, such as skill level. One way to test this hypothesis is by categorizing salary into three levels (low, moderate, and high) and skills sets into two levels (entry level...
13.8K
Design Example: Designing a Residential Plumbing System01:25

Design Example: Designing a Residential Plumbing System

1.1K
The design of residential plumbing systems requires carefully evaluating water demand, flow rates, and pressure dynamics to ensure both efficiency and reliability. The nature of water flow within pipes is defined by its Reynolds number, which classifies flow as either laminar (smooth) or turbulent.
1.1K
Design Example: Designing Water Slide01:18

Design Example: Designing Water Slide

630
When designing a water slide, controlling the speed of water flow is crucial for rider safety while maintaining an exciting experience. As water flows down the slide, gravity causes it to accelerate, with its speed at the bottom depending on the height from which it starts. The higher the slide, the more potential energy the water has at the top, which is converted into kinetic energy as it descends, increasing its speed.
Bernoulli's principle determines the water's velocity along the slide....
630
Design Example: Design of an Irrigation Channel01:27

Design Example: Design of an Irrigation Channel

843
Trapezoidal channels are widely used in irrigation systems due to their cost-effectiveness and efficiency in conveying water. Trapezoidal channels feature a flat bottom and sloping sides, making them stable and easier to construct compared to other shapes. The bottom width and side slope ratio are determined based on the required flow capacity and site conditions. The side slope is kept gentle for unlined channels to prevent soil erosion.Hydraulic parameters in channel design include the flow...
843
Design Example01:23

Design Example

553
The innovation of touch-tone telephony revolutionized the telecommunications industry by replacing the traditional rotary dial with a dual-tone multi-frequency (DTMF) signaling system. This system uses a matrix-style keypad with buttons arranged in four rows and three columns, creating 12 distinct signals each assigned to a pair of frequencies. Each button press results in a simultaneous generation of two sinusoidal tones – one from a low-frequency group (697 to 941 Hz) and one from a...
553

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing the Cytotoxicity and Apoptotic Efficacy of Parasporin-2-Derived Variants (Mpp46Aa1) on Cancer Cell Lines.

Toxins·2024
Same author

Comprehensive Analysis of Kisspeptin Signaling: Effects on Cellular Dynamics in Cervical Cancer.

Biomolecules·2024
Same author

Aberrant <i>AHRR</i>, <i>ADAMTS2</i> and <i>FAM184</i> DNA Methylation: Candidate Biomarkers in the Oral Rinse of Heavy Smokers.

Biomedicines·2023
Same author

Toxic Determination of Cry11 Mutated Proteins Obtained Using Rational Design and Its Computational Analysis.

International journal of molecular sciences·2023
Same author

Comparative Virucidal Activities of Essential Oils and Alcohol-Based Solutions against Enveloped Virus Surrogates: In Vitro and In Silico Analyses.

Molecules (Basel, Switzerland)·2023
Same author

Chemoinformatics and artificial intelligence colloquium: progress and challenges in developing bioactive compounds.

Journal of cheminformatics·2022
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Feb 5, 2026

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
10:51

Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

Published on: October 31, 2014

27.9K

Softepigen: Primers Design Web-Based Tool for MS-HRM Technique.

Efrain Pinzon-Reyes, William Armando Alvarez, Paola Rondon-Villarreal

    IEEE/ACM Transactions on Computational Biology and Bioinformatics
    |September 4, 2018
    PubMed
    Summary
    This summary is machine-generated.

    Softepigen is the first software for designing primers for Methylation Sensitive High-Resolution Melting (MS-HRM) analysis. This tool enhances DNA methylation assessment by ensuring primer specificity in genomic regions.

    More Related Videos

    Fabrication and Design of Wood-Based High-Performance Composites
    08:08

    Fabrication and Design of Wood-Based High-Performance Composites

    Published on: November 9, 2019

    14.0K
    Primer-Free Aptamer Selection Using A Random DNA Library
    11:14

    Primer-Free Aptamer Selection Using A Random DNA Library

    Published on: July 26, 2010

    25.4K

    Related Experiment Videos

    Last Updated: Feb 5, 2026

    Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
    10:51

    Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo

    Published on: October 31, 2014

    27.9K
    Fabrication and Design of Wood-Based High-Performance Composites
    08:08

    Fabrication and Design of Wood-Based High-Performance Composites

    Published on: November 9, 2019

    14.0K
    Primer-Free Aptamer Selection Using A Random DNA Library
    11:14

    Primer-Free Aptamer Selection Using A Random DNA Library

    Published on: July 26, 2010

    25.4K

    Area of Science:

    • Epigenetics
    • Bioinformatics
    • Molecular Biology

    Background:

    • DNA methylation analysis is crucial for understanding gene regulation.
    • Methylation Sensitive High-Resolution Melting (MS-HRM) is a PCR-based technique for DNA methylation assessment.
    • Designing specific primers for MS-HRM is challenging and lacks automated software solutions.

    Purpose of the Study:

    • To develop the first comprehensive software for automated MS-HRM primer design.
    • To provide a tool that adheres to established primer design recommendations for MS-HRM.
    • To enhance the specificity of primer binding in human genomic sequences.

    Main Methods:

    • Development of Softepigen, a novel primer design software for MS-HRM.
    • Implementation of Wojdacz's recommendations for primer selection.
    • Targeting genomic regions with high linguistic complexity for increased specificity.
    • In-silico PCR analysis using BiSearch ePCR tool for primer validation.

    Main Results:

    • Softepigen successfully designs primers for MS-HRM analysis.
    • In-silico validation confirmed satisfactory specificity of the designed primers.
    • The software targets primer binding sites with high linguistic complexity sequences.
    • The developed primers showed good performance in selected genomic regions.

    Conclusions:

    • Softepigen is a valuable and the first available tool for automated MS-HRM primer design.
    • The software enhances the specificity and reliability of DNA methylation assessment.
    • Softepigen is freely accessible online at www.soft-epigen.com for researchers.