Related Experiment Video
Updated: Feb 5, 2026
Cochlear Implantation in the Guinea Pig
Published on: June 15, 2018
In Vitro and In Vivo (Rabbit, Guinea Pig, Mouse) Properties of a Novel Resorbable Polymer and Allogenic Bone
G Gut1, M Ambroziak2, W Bojar3
1Department of Transplantology and Central Tissue Bank, Medical University of Warsaw, Warsaw, Poland.
Introduction:
Currently, there is no bone fixation material that could be fully replaced by the competent recipient bone. The creeping substitution of the bone graft by the recipient bone is the result of its unique potential related to the presence of bone morphogenetic proteins (BMPs). However, the size of the human bone limits the use of allogenic implants for surgical (orthopedic) fixation. The aim of this project was to develop a novel composite material for guided bone regeneration, consisting of human bone powder obtained from a tissue bank and a resorbable polymer (13 wt% of bone powder in a medical poly-l-lactide polymer). Such a biomaterial could possess osteoinductive properties and be used to manufacture bone fixation implants of different shapes and sizes.
Materials And Methods:
The samples were obtained by tape casting and foils pressing, and subsequently radiation sterilized with a dose of 35 kGy. Two cell lines-normal mouse embryo fibroblasts (Balb 3T3/c) and human fetal osteoblasts (hFOB 1.19)-were cultured with the extracts of the biomaterials (MTT assay) or in indirect contact with the evaluated biomaterials (agar diffusion method). In addition, cell viability was evaluated after 5 days of incubation with biomaterial using ThinCert tissue culture inserts. Then, the following in vivo examinations were conducted: acute systemic toxicity, skin irritation and sensitization, and local effects after implantation.
Results:
The evaluated composite material showed a high degree of cytocompatibility and biocompatibility according to the International Standards.
Conclusions:
The preclinical evaluation we performed on the new, polylactide-based allogenic biomaterial opens up possibilities to patent pending and advanced in vivo testing.
More Related Videos
08:41Calvarial Model of Bone Augmentation in Rabbit for Assessment of Bone Growth and Neovascularization in Bone Substitution Materials
Published on: August 13, 2019
05:14Author Spotlight: Extraction of Guinea Pig Round Window Membrane to Facilitate Inner Ear Drug Delivery Research
Published on: February 23, 2024
Related Concept Videos
01:55Bone Structure
01:40Bone Remodeling
01:27Compact Bone
Compact bone, also called cortical bone, is the denser, stronger of the two types of bone tissue. It is found under the periosteum and in the diaphyses of long bones, where it provides support and protection. The microscopic structural unit of compact bone is called an osteon, or haversian system. Each osteon is composed of concentric rings of calcified...
01:29Bone Disorders
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
01:12The Hyoid Bone
01:09Spongy Bone
Spongy bone is more porous, and less dense compared to compact bone. It is composed of concentric lamellae that are arranged irregularly to form the trabecular network. In some bones, the spaces between trabeculae contain red marrow, where...