Related Experiment Video
Updated: Dec 10, 2025
Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
Comparison of mouse, rat and rabbit models for adipose - Derived stem cells (ASC) research
A Zolocinska1, K Siennicka1, T Debski1
1Department of Regenerative Medicine, Maria Sklodowska-Curie National Research Institute of Oncology, Warsaw, Poland.
Purpose Of The Study:
Cellular therapies are becoming more popular and there is a big demand for suitable animal model for research in field of tissue engineering. Both the small (rodents) and large animals have their advantages for cellular therapy experiments. Appropriate animal research model would be a bridge between basic research and clinical medicine. The aim of this study was to compare mouse, rat and rabbit as animal models useful for adipose - derived stem cell research.
Materials And Methods:
Quantity, phenotype, clonogenic and differentiation potential of cells isolated from different localizations of adipose tissue from WAG and LEW/W rat strains, rabbit and mouse were analysed.
Results:
The highest number of cells from 1 g of tissue were isolated from cervical white fat of LEW/W rat. ASCs isolated from rat had also the highest clonogenic potential. Phenotype and capability to differentiate into osteogenic, adipogenic and chondrogenic lineages are at the same level for rat and rabbit.
Conclusions:
Rat as a research model can be a rational solution between large animal models and typical laboratory mice because of their size, genetic homogenity, availability of genetically modified stains and possibility to perform research mimicking clinical applications.
More Related Videos
10:31An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment
Published on: April 20, 2018
08:09Isolation of Rat Adipose Tissue Mesenchymal Stem Cells for Differentiation into Insulin-producing Cells
Published on: August 29, 2022