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Dual Raster-Scanning Photoacoustic Small-Animal Imager for Vascular Visualization
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High-speed raster-scanning synchrotron serial microcrystallography with a high-precision piezo-scanner
Yuan Gao1, Weihe Xu1, Wuxian Shi1
1Photon Sciences, Brookhaven National Laboratory, Upton, NY 11973, USA.
Journal of Synchrotron Radiation
|September 5, 2018
Summary
A new high-speed goniometer enables faster serial microcrystallography at the Frontier Microfocus Macromolecular Crystallography (FMX) beamline. This advancement significantly reduces data collection time, maximizing the use of powerful synchrotron light sources.
Area of Science:
- Structural Biology
- Crystallography
- Synchrotron Radiation
Background:
- The Frontier Microfocus Macromolecular Crystallography (FMX) beamline offers high photon flux and a small beam size, ideal for advanced structural biology.
- High dose rates at FMX beamlines present opportunities for accelerating serial microcrystallography experiments.
Purpose of the Study:
- To develop and validate a high-speed, high-precision goniometer for serial microcrystallography at the FMX beamline.
- To enable measurements utilizing the full flux capabilities of the FMX beamline.
Main Methods:
- Design and construction of a novel XYZ piezo positioner-based goniometer.
- Characterization of goniometer precision and speed using laboratory and serial crystallography measurements.
- Testing performance up to 750 Hz frame rate using Eiger 16M detector's region-of-interest mode.
Main Results:
- The piezo-based goniometer achieves sub-100 nm raster-scanning precision at frequencies over 10 grid-linepairs/s.
- Experimental speeds were verified in laboratory and serial crystallography setups.
- The system demonstrated performance at the maximum frame rate of the detector's ROI mode.
Conclusions:
- The developed goniometer significantly reduces serial crystallography data collection time.
- This instrumentation allows for the full utilization of high-brightness synchrotron radiation facilities.
- Enables unprecedented experimental speeds for structural biology research.
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