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[Interferon sensitivity of various cell lines].

M Tonew, B Glück

    Journal of Basic Microbiology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Determining interferon (IFN) yield requires sensitive cell lines. Human fibroblasts and MDBK cells are most effective for interferon assays, while FL and Vero cells are unsuitable.

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    Area of Science:

    • Virology
    • Cell Biology
    • Immunology

    Context:

    • Accurate quantification of interferon (IFN) is crucial for its induction, production, and purification.
    • Assessing the susceptibility of various cell lines to interferon is essential for developing reliable bioassays.
    • The study utilized a microtitre system measuring the inhibition of cytopathic effect caused by vesicular stomatitis virus.

    Purpose:

    • To compare the sensitivity of different cell lines for quantifying interferon (IFN) alpha yield.
    • To identify optimal cell lines for interferon bioassays using an internal IFN alpha standard.
    • To evaluate the suitability of commonly used and novel cell lines for interferon yield determination.

    Summary:

    • Human fibroblasts with trisomy G 21 and bovine MDBK cells demonstrated the highest sensitivity in interferon assays.

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  • Normal human fibroblasts also showed significant sensitivity, followed by human RH kidney cells and human WISH amnion cells.
  • Human amnion FL cells and monkey Vero kidney cells proved unsuitable for accurate interferon yield determination.

    • Impact:

    • Identifies superior cell lines (human fibroblasts, MDBK) for sensitive and reliable interferon quantification.
    • Provides guidance for researchers selecting cell lines in interferon research and development.
    • Highlights the limitations of certain cell lines (FL, Vero) in interferon bioassays, preventing potential inaccuracies.