Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell-surface Signaling01:21

Cell-surface Signaling

54.5K
Hormones—or any molecule that binds to a receptor, known as a ligand—that are lipid-insoluble (water-soluble) are not able to diffuse across the cell membrane. In order to be able to affect a cell without entering it, these hormones bind to receptors on the cell membrane. When a first messenger, a hormone, binds to a receptor, a signal cascade is set off, causing second messengers, proteins inside the cell, to become activated, resulting in downstream effects.
54.5K
Skeletal Muscle Relaxants: Adverse Effects01:21

Skeletal Muscle Relaxants: Adverse Effects

843
Skeletal muscle relaxants are widely used for muscle paralysis and relieving pain following any muscle injury or stiffness. However, depending on the drug type, they can have adverse effects that range from mild to severe. Usually, nondepolarizing neuromuscular blockers have minimal side effects. For example, drugs like d-tubocurarine, cisatracurium, and rocuronium cause hypotension, whereas drugs like baclofen, when stopped abruptly, can lead to the recurrence of spastic conditions.
Unlike...
843
Factors Affecting Dissolution: Particle Size and Effective Surface Area01:23

Factors Affecting Dissolution: Particle Size and Effective Surface Area

1.7K
Dissolution kinetics, an essential aspect of oral drug delivery, is significantly influenced by the drug's particle size. According to the Noyes-Whitney dissolution model, the dissolution rate correlates directly with the drug's surface area. The larger the surface area, the higher the drug's solubility in water, leading to a faster drug dissolution rate. Reducing particle size increases the effective surface area, enhancing the dissolution process. Micronization and nanosizing are...
1.7K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.3K
Overview
80.3K
iPS Cell Differentiation01:22

iPS Cell Differentiation

3.1K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
3.1K
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

16.7K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
16.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Corrigendum to "Multifunctional piezoelectric surfaces enhanced with layer-by-layer coating for improved osseointegration and antibacterial performance" [Colloids Surf. B: Biointerfaces 243 (2024) 114123].

Colloids and surfaces. B, Biointerfaces·2026
Same author

Remote control of protein deposition on magnetoactive composites.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Pediatric papillary thyroid carcinoma: postoperative management and 14-year experience with radioiodine therapy at a Portuguese oncology centre.

European journal of pediatrics·2026
Same author

Ovary-Derived Signals Align Protein Appetite with Oogenesis.

bioRxiv : the preprint server for biology·2026
Same author

LIPOHYPERTHROPHY: NEW TECHNOLOGY, OLD PROBLEMS.

Acta endocrinologica (Bucharest, Romania : 2005)·2026
Same author

Impact of a centralised pancreaticobiliary tumour board on the diagnosis of pancreatic lesions.

Acta gastro-enterologica Belgica·2026

Related Experiment Video

Updated: Feb 5, 2026

Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.5K

Electroactive biomaterial surface engineering effects on muscle cells differentiation.

S Ribeiro1, A C Gomes2, I Etxebarria3

  • 1Centro de Física, Universidade do Minho, 4710-057 Braga, Portugal; Centre of Molecular and Environmental Biology (CBMA), Universidade do Minho, Campus de Gualtar, 4710-057 Braga, Portugal.

Materials Science & Engineering. C, Materials for Biological Applications
|September 7, 2018
PubMed
Summary

Poly(vinylidene fluoride) (PVDF) biomaterials enhance skeletal muscle regeneration by mimicking natural cues. Charged surfaces and oriented fibers promote myoblast differentiation and cell alignment, offering a promising approach for muscle tissue engineering.

Keywords:
DifferentiationMyoblast cellsPVDFPiezoelectricTissue engineering

More Related Videos

Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.6K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.7K

Related Experiment Videos

Last Updated: Feb 5, 2026

Engineering a Bilayered Hydrogel to Control ASC Differentiation
07:48

Engineering a Bilayered Hydrogel to Control ASC Differentiation

Published on: May 25, 2012

14.5K
Accessing the Cytotoxicity and Cell Response to Biomaterials
09:46

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

4.6K
Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering
12:22

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

Published on: March 1, 2016

8.7K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Severe skeletal muscle injuries lead to irreversible cell loss and functional deficits.
  • Current cell therapies are insufficient for treating significant muscle damage.
  • Biomaterials like poly(vinylidene fluoride) (PVDF) offer potential for muscle regeneration.

Purpose of the Study:

  • To investigate how PVDF surface properties (charge and morphology) influence myoblast differentiation.
  • To evaluate PVDF's potential for skeletal muscle tissue engineering.

Main Methods:

  • Utilized C2C12 myoblasts cultured on PVDF films and electrospun fibers with varying surface charges and orientations.
  • Assessed myoblast differentiation through quantitative analysis of myotube fusion, maturation, length, diameter, and cell alignment.
  • Examined the impact of piezoelectric and ferroelectric properties of PVDF on cell behavior.

Main Results:

  • PVDF significantly promoted myogenic differentiation of C2C12 cells.
  • Charged PVDF surfaces enhanced muscle cell fusion and myotube maturation.
  • Oriented PVDF electrospun fibers promoted cell alignment, unlike randomly oriented fibers.

Conclusions:

  • PVDF biomaterials effectively support skeletal muscle regeneration and myoblast differentiation.
  • Surface charge and fiber orientation are critical factors in PVDF's efficacy for muscle tissue engineering.
  • Electroactive PVDF offers a viable strategy for creating microenvironments for enhanced muscle repair.