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Raman Spectroscopy: Overview01:20

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Surfactant-Free Interface Suspended Gold Graphitic Surface-Enhanced Raman Spectroscopy Substrate for Simultaneous

Liang Zhang1, Fang Liu1, Yuxiu Zou1

  • 1Molecular Sciences and Biomedicine Laboratory, State Key Laboratory for Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Innovation Center for Molecular Engineering and Theranostics , Hunan University , Changsha , Hunan 410082 , China.

Analytical Chemistry
|September 7, 2018
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Summary
This summary is machine-generated.

This study introduces a novel graphene-isolated-gold-nanocrystal substrate for simultaneous multiphase analysis. This surfactant-free system enables sensitive detection and enrichment of lipid- and water-soluble drugs, advancing pharmacokinetic studies.

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Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biomedical Engineering

Background:

  • Simultaneous detection of multiplex analytes with varying solubility is crucial for pharmaceutical analysis, particularly for synergistic therapies.
  • Surface-enhanced Raman spectroscopy (SERS) offers sensitive, label-free detection but faces limitations in multiphase applications due to substrate modification requirements.
  • Existing SERS methods often require surfactants or inducers, hindering broad application in complex biological systems.

Purpose of the Study:

  • To develop an innovative SERS substrate for simultaneous multiphase analysis of multiplex analytes.
  • To overcome the limitations of traditional SERS methods by eliminating the need for surfactants or surface modification.
  • To demonstrate the utility of the developed substrate for ex vivo pharmacokinetic analysis of co-administered drugs.

Main Methods:

  • Fabrication of a graphene-isolated-gold-nanocrystal SERS substrate capable of self-suspension at multiphase interfaces.
  • Utilizing the unique vibration band of the graphitic shell as an internal standard for enhanced quantification accuracy.
  • Performing ex vivo experiments with mimic lipid- and water-soluble drugs in mice to validate multiphase enrichment and detection capabilities.

Main Results:

  • The developed gold graphitic SERS substrate spontaneously suspends at interfaces without surfactants, ensuring analyte proximity.
  • The substrate enables simultaneous enrichment and sensitive SERS detection of analytes from different phases without interference.
  • Accurate quantification was achieved using the graphitic shell's vibration band as an internal standard, validated in ex vivo drug analysis.

Conclusions:

  • A novel, surfactant-free SERS substrate facilitates simultaneous multiphase analysis and enrichment.
  • The substrate's design enhances SERS quantification accuracy through an intrinsic internal standard.
  • This technology shows significant potential for simultaneous multiplex pharmacokinetic analysis of co-administered drugs.