EFFICACY OF MESENCHYMAL STEM CELLS USED FOR THE IMPROVEMENT CEREBRAL MICROCIRCULATION IN SPONTANEOUSLY HYPERTENSIVE...

Area of Science:

• Neuroscience
• Regenerative Medicine
• Cardiovascular Biology

Background:

• Persistent high blood pressure causes brain microcirculatory disorders.
• Developing new correction methods is crucial for medicine and biology.
• Spontaneously hypertensive rats (SHR) are a model for studying hypertension-related brain changes.

Purpose of the Study:

• To investigate the effect of intracerebral human mesenchymal stem cells (MSCh) transplantation on cerebral microcirculation.
• To compare the efficacy of MSCh transplantation in young and aged spontaneously hypertensive rats (SHR).

Main Methods:

• Intracerebral transplantation of human mesenchymal stem cells (MSCh).
• Assessment of microvascular network density in young (4 months) and aged (12 months) SHR.
• Evaluation of microvascular network density in the pia mater's arteriolar area.
• Measurement of sensorimotor cortex perfusion and tissue saturation.

Main Results:

• MSCh transplantation increased microvascular network density in young SHR by approximately 1.6-fold.
• The density of the arteriolar area in the pia mater's microvascular network increased by approximately 1.9-fold in young SHR.
• MSCh transplantation increased microvascular network density in aged SHR by approximately 1.4–1.5-fold.
• Perfusion and tissue saturation in the sensorimotor cortex of young SHR reached levels comparable to normotensive rats.
• Perfusion and tissue saturation in the sensorimotor cortex of aged SHR showed no significant increase.

Conclusions:

• Intracerebral MSCh transplantation largely reversed microcirculatory damage in the young SHR brain.
• The treatment provided only minor improvements in cerebral microcirculation for aged SHR, suggesting age-dependent efficacy.