ITLN1, PPARg AND TNFa GENE EXPRESSION IN VISCERAL ADIPOSE TISSUE

Area of Science:

• Endocrinology
• Molecular Biology
• Metabolic Disease Research

Background:

• Adipose tissue functions as an endocrine organ, crucial in regulating gene expression for obesity and related comorbidities like diabetes and cardiovascular disease.
• Understanding tissue-specific gene regulation in adipose tissue is vital for metabolic health research.

Purpose of the Study:

• To investigate the expression of ITLN1, PPARγ, and TNFα genes in intra-abdominal adipose tissue.
• To determine the effect of these gene expressions on serum omentin 1 and TNFα levels in individuals with varying body mass indexes (BMI).

Main Methods:

• Analysis of ITLN1, PPARγ, and TNFα gene expression in intra-abdominal adipose tissue.
• Measurement of serum omentin 1 and TNFα levels.
• Correlation analysis between gene expression, protein levels, and serum markers across different BMI groups.

Main Results:

• Serum TNFα levels were significantly higher in overweight and obese individuals (BMI ≥ 25 kg/m²) compared to normal weight individuals (BMI < 25 kg/m²).
• PPARγ gene expression positively correlated with ITLN1 gene expression in intra-abdominal adipose tissue.
• Serum omentin 1 levels positively correlated with PPARγ mRNA and protein levels.
• In overweight/obese individuals, TNFα mRNA expression negatively correlated with PPARγ and ITLN1 mRNA levels.

Conclusions:

• This study establishes a novel correlation between PPARγ gene expression in intra-abdominal adipose tissue and the expression/secretion levels of omentin 1.
• Findings highlight the intricate relationship between specific gene expressions in adipose tissue and systemic markers of inflammation and metabolic status.
• The results contribute to understanding the molecular mechanisms underlying obesity and its comorbidities.