COMPARISON OF THE SHAPE OF RABBIT MESENCHYMAL STROMAL CELLS DURING FIVE PASSAGES AFTER PRODUCTION OF PRIMARY CULTURE

Area of Science:

• Cell biology
• Stem cell research
• Tissue engineering

Background:

• Cell culture requires artificial conditions, leading to loss of macroorganism control and increased modification risk.
• Limited data exists on early indicators and timing of cell modifications during culture.
• Mesenchymal stromal cells (MSCs) are crucial for regenerative medicine and require careful cultivation.

Purpose of the Study:

• To investigate early modifications in rabbit medullary mesenchymal stromal cells (MSCs) during serial passaging.
• To evaluate the impact of passaging on cell area and spreading coefficient.
• To identify potential indicators of cellular changes in cultured MSCs.

Main Methods:

• Rabbit medullary mesenchymal stromal cells (MSCs) were cultured and passaged up to 5 times.
• Cell area and spreading coefficient were measured 1 hour and 1 day post-reseeding.
• Digital images of living cells were analyzed to quantify morphological changes.

Main Results:

• Absolute cell area increased with successive passages.
• The increment in cell growth area was independent of the passage number.
• Cell populations shifted from predominantly smaller cells in early passages to larger cells in later passages.
• The spreading coefficient remained unaffected by passaging.

Conclusions:

• Cell passaging influences MSC size, with a notable increase in larger cells over time.
• Cell area changes can serve as an indicator of modifications during MSC cultivation.
• The spreading coefficient is not a reliable indicator of passaging-induced modifications in these cells.