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Updated: Feb 5, 2026

A Mouse Model for Laser-induced Choroidal Neovascularization
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A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

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Amount of Mononuclear Phagocyte Infiltrate Does Not Predict Area of Experimental Choroidal Neovascularization (CNV)

Adrian Will-Orrego1, Yubin Qiu1, Elizabeth S Fassbender1

  • 1Department of Ophthalmology, Novartis Institutes for Biomedical Research , Cambridge, Massachusetts.

Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics
|September 7, 2018
PubMed
Abstract

Insights

Mononuclear phagocytes (MNPs) infiltrate choroidal neovascularization (CNV) lesions. Therapies targeting CNV angiogenesis did not alter MNP infiltration, suggesting MNP number is independent of lesion size.

Area of Science:

  • Ophthalmology
  • Immunology
  • Angiogenesis research

Background:

  • Mononuclear phagocytes (MNPs) are key players in neovascular age-related macular degeneration (nv AMD), also known as choroidal neovascularization (CNV).
  • The density and characteristics of MNPs within CNV lesions are influenced by the local microenvironment and therapeutic interventions.
  • Understanding MNP behavior is crucial for developing effective treatments for AMD.

Purpose of the Study:

  • To investigate ocular cell infiltration, specifically MNPs, in a laser-induced mouse model of CNV.
  • To explore conditions that modulate angiogenesis and MNP infiltration in the context of CNV.
  • To assess the impact of toll-like receptor-2 (TLR-2) activation, anti-VEGF therapy, and Ccl2 deletion on MNP dynamics.

Main Methods:

  • Developed and utilized specific assays to quantify MNP numbers in subretinal spaces and within CNV lesions in a mouse model.
  • Administered a TLR-2 activating peptide to assess its effect on CNV and MNP infiltration.
  • Utilized anti-VEGF therapy and Ccl2 genetic deletion to evaluate their influence on angiogenesis and MNP infiltration.

Main Results:

  • Laser injury successfully induced CNV and subsequent MNP infiltration.
  • TLR-2 activation significantly increased CNV area, MNP numbers, and MNP density.
  • Anti-VEGF therapy reduced CNV area, while Ccl2 deletion increased it; however, MNP infiltration remained unchanged in both cases.
  • Surprisingly, MNP infiltration levels did not correlate with the extent of CNV angiogenesis under anti-VEGF or Ccl2-deleted conditions.

Conclusions:

  • Quantification of MNPs offers valuable biological insights for potential AMD therapies.
  • The number of infiltrating MNPs does not appear to directly correlate with the size of laser-induced CNV lesions.
  • These findings suggest that MNP infiltration may be regulated independently of the degree of angiogenesis in CNV.
Keywords:
CNVMNPsTLR-2angiogenesisanti-VEGFmicroglia

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