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Updated: Feb 5, 2026
A Rapid Image-based Bacterial Virulence Assay Using Amoeba
Published on: June 27, 2018
Abstract:
In this paper, the protocol which we have developed to get satisfactory spreads of Amoeba proteus mitotic chromosomes is presented, and the process of karyotyping this amoeba species is described. This protocol allows obtaining of extended chromosomic with repeatable chromomeres pattern in individual chromosomes. We have shown that metaphase in «B»-strain amoebae (one of the type strains of A. proteus in the Amoebae Strains Collection at the Institute of Cytology) contains 27 chromosome pairs with homologous chromomeric pattern. It is ascertained that chromomeric pattern is chromosome-specific feature. The bank of images of DAPI- and YoYo1-banded individual metaphase chromosomes of «B»-strain of A. proteus is presented.
Insights
A new protocol for spreading Amoeba proteus mitotic chromosomes enables detailed karyotyping. This method reveals 27 chromosome pairs with unique, repeatable banding patterns, aiding in species identification.
Area of Science:
- Cell Biology
- Genetics
- Protistology
Background:
- Karyotyping Amoeba proteus is challenging due to chromosome structure.
- Standardized protocols for mitotic chromosome preparation are lacking for this species.
Purpose of the Study:
- To present a novel protocol for obtaining extended mitotic chromosome spreads of Amoeba proteus.
- To describe the karyotyping process for A. proteus using this protocol.
- To characterize the chromomeric patterns of A. proteus chromosomes.
Main Methods:
- Development of a chromosome spreading protocol for Amoeba proteus.
- Karyotyping using DAPI and YoYo1 staining.
- Analysis of chromomeric patterns in metaphase chromosomes.
Main Results:
- A repeatable protocol yielding extended A. proteus mitotic chromosomes was established.
- The 'B'-strain of A. proteus possesses 27 chromosome pairs.
- Chromosome-specific, homologous chromomeric patterns were identified and documented in an image bank.
Conclusions:
- The developed protocol facilitates detailed karyotyping of Amoeba proteus.
- Chromomeric patterns are reliable, chromosome-specific identifiers for A. proteus.
- This work provides a valuable resource for A. proteus research and strain identification.
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