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Related Experiment Video

Updated: Feb 5, 2026

Method to Produce Durable Pellets at Lower Energy Consumption Using High Moisture Corn Stover and a Corn Starch Binder in a Flat Die Pellet Mill

Method to Produce Durable Pellets at Lower Energy Consumption Using High Moisture Corn Stover and a Corn Starch Binder in a Flat Die Pellet Mill

Published on: June 15, 2016

Enzyme Pretreatment Enhancing Biogas Yield from Corn Stover: Feasibility, Optimization, and Mechanism Analysis.

Siqi Wang^1,2, Fan Li¹, Dan Wu¹

¹Beijing Key Lab for Source Control Technology of Water Pollution , Beijing Forestry University , Beijing 100083 , China.

Journal of Agricultural and Food Chemistry

|September 8, 2018

Summary

Enzyme pretreatment significantly boosts biogas production from corn stover by improving its structure and digestibility. Optimal conditions increase biogas yield by 36.9% and speed up the anaerobic digestion process.

Keywords:

biogas production corn stover enzyme pretreatment kinetic analysis lignocellulosic structure

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Ammonia Fiber Expansion AFEX Pretreatment of Lignocellulosic Biomass

Ammonia Fiber Expansion AFEX Pretreatment of Lignocellulosic Biomass

Published on: April 18, 2020

Area of Science:

Biochemical Engineering
Renewable Energy
Biomass Conversion

Background:

Corn stover is a promising lignocellulosic biomass for biofuel production.
Anaerobic digestion (AD) is a key technology for converting biomass into biogas.
Enhancing AD efficiency of corn stover is crucial for sustainable energy generation.

Purpose of the Study:

To investigate the feasibility, optimization, and mechanisms of enzyme pretreatment for enhancing corn stover anaerobic digestion.
To determine the optimal enzyme pretreatment conditions for maximizing biogas yield.
To understand how enzyme pretreatment affects the structural and compositional properties of corn stover.

Main Methods:

Enzyme pretreatment of corn stover using varying enzyme loads, pretreatment times, and solid content.
Anaerobic digestion of pretreated and untreated corn stover in batch reactors.
Analysis of biogas yield, hydrolysis rate, biogas production rate, and soluble chemical oxygen demand (SCOD).
Kinetic and correlation analyses to evaluate pretreatment effects.

Main Results:

Enzyme pretreatment significantly enhanced cumulative biogas yield by 36.9% under optimal conditions (30 FPU/g enzyme load, 24 h, 60 g/L solid content).
Pretreatment led to corn stover surface structure disruption and non-crystalline cellulose degradation, improving biodegradability.
Enzyme pretreatment accelerated hydrolysis rate (0.15/d) and biogas production rate (23.89 mL/gVS), and reduced lag phase to 1.2 d.
An SCOD yield of 250-350 mg/g was identified as optimal for subsequent anaerobic digestion.

Conclusions:

Enzyme pretreatment is a feasible and effective method to enhance anaerobic digestion of corn stover.
Optimal pretreatment conditions significantly improve biogas yield and process kinetics.
Understanding the mechanisms, including structural changes and SCOD generation, is key to optimizing biomass-to-biogas conversion.