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Interaction of immunosuppressive agents and human adenovirus infection in mice
Abstract:
It was examined whether sensitivity of mice to human adenovirus infections is affected by immunosuppressive agents. Mice treated with the lymphotropic cytostatic dianhydrodulcitol (DAD) or antilymphocytic serum (ALS) did not become susceptible to human adenovirus type 12 infection, as the virus did not replicate--not even in its component forms--in the animals. On the other hand, the effect of both DAD and ALS on the lymphoid organs of mice was intensified by human adenovirus infection. In tissue cultures the reproduction of adenovirus was facilitated by DAD.
Insights
Immunosuppressive agents like dianhydrodulcitol (DAD) and antilymphocytic serum (ALS) did not increase mouse susceptibility to human adenovirus infections. However, adenovirus infection intensified the effects of DAD and ALS on mouse lymphoid organs.
Area of Science:
- Virology
- Immunology
- Pharmacology
Background:
- Human adenoviruses are common pathogens.
- Immunosuppressive agents can alter host-pathogen interactions.
- The impact of immunosuppression on adenovirus susceptibility in mice is not fully understood.
Purpose of the Study:
- To investigate the effect of immunosuppressive agents on mouse susceptibility to human adenovirus type 12.
- To examine the reciprocal effect of human adenovirus infection on immunosuppressive agents in mice.
Main Methods:
- Mice were treated with dianhydrodulcitol (DAD) or antilymphocytic serum (ALS).
- Mice were infected with human adenovirus type 12.
- Viral replication and effects on lymphoid organs were assessed.
- Adenovirus reproduction in tissue cultures with DAD was evaluated.
Main Results:
- Mice treated with DAD or ALS did not become susceptible to human adenovirus type 12 infection.
- Human adenovirus infection intensified the effects of DAD and ALS on mouse lymphoid organs.
- DAD facilitated adenovirus reproduction in tissue cultures.
Conclusions:
- Immunosuppressive agents DAD and ALS do not confer susceptibility to human adenovirus type 12 in mice.
- Human adenovirus infection exacerbates the immunosuppressive effects of DAD and ALS on lymphoid organs.
- DAD enhances adenovirus replication in vitro.