THE COMPOSITION OF SWI/SNF CHROMATIN REMODELING COMPLEX IS STABLE DURING GENE TRANSCRIPTION

SWI/SNF protein complex is a unique transcription activator, which takes part in almost all stages of transcription regulation including preinitiation complex formation, transition of RNA polymerase II to transcription elongation, enhancer and insulator action. Recently it was found that particular subunits of SWI/SNF become involved in gene transcription on distinct stages. In the current work we investigate subunit composition of complexes formed with Brahma protein (ATPase of SWI/SNF complex) on the sages of initiation and elongation of gene transcription. As the result we have demonstrated the composition stability of SWI/SNF complex during gene transcription. We suppose that differences in recruitment of particular SWI/SNF subunits, that were distinguished earlier, are due to formation of complexes distinct from SWI/SNF and not including Brahma ATPase. Functioning of non-enzymatic subunits independently of the main complex had not been shown earlier. This finding extend our knowledge concerning functional properties of the proteins, involved in chromatin remodeling process.