[THE PERMEABILITY OF DURA MATER OF SPINAL CORD CANINE FOR LOW-MOLECULAR AGENTS OF BLOOD SERUM]

Area of Science:

• Neuroscience
• Biochemistry
• Physiology

Background:

• The dura mater (DM) acts as a protective barrier for the central nervous system.
• Understanding DM permeability is crucial for drug delivery and understanding neuro-inflammatory processes.

Purpose of the Study:

• To quantify the in vitro permeability of canine spinal cord dura mater to various low- and high-molecular weight blood serum components.
• To investigate the relationship between substrate concentration and DM permeability.
• To correlate DM structural features with its observed permeability characteristics.

Main Methods:

• In vitro study of canine spinal cord dura mater.
• Measurement of component permeability using techniques not specified, likely involving diffusion assays.
• Evaluation of permeability for urea, creatinine, glucose, lactate, cholesterol, calcium, inorganic phosphate, and albumin.

Main Results:

• Most studied components exhibited DM permeability between 8% and 15%.
• Lactate demonstrated the highest permeability (33.6%), whereas cholesterol showed the lowest (1.3%).
• Non-linear permeability dependence on initial substrate concentration was observed for urea and creatinine, with threshold permeabilities of 4.83 mmol/L and 97 μmol/L, respectively.

Conclusions:

• Canine spinal cord dura mater exhibits selective permeability to blood serum components.
• Structural features, including dense fibrillar packing and high sulfated glycosaminoglycan content, likely dictate DM permeability.
• These findings have implications for understanding the transport of substances across the blood-brain barrier in the spinal cord.