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Related Experiment Video

Updated: Feb 5, 2026

Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
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[ROLE OF MITOCHONDRIAL REACTIVE OXYGEN SPECIES IN LIPOPOLYSACCHARIDE-PROMOTED ACCUMULATION OF INTRACELLULAR LIPID

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    Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova
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    Bacterial lipopolysaccharide (LPS) triggers mitochondrial dysfunction and lipid droplet accumulation in epithelial cells. Mitochondrial reactive oxygen species (mROS) are key mediators of this LPS-induced neutral lipid deposition.

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    Area of Science:

    • Cell Biology
    • Mitochondrial Biology
    • Lipid Metabolism

    Background:

    • Bacterial lipopolysaccharide (LPS) is known to induce mitochondrial dysfunction and triglyceride accumulation in various cell types.
    • The exact mechanisms underlying LPS-induced neutral lipid deposition are not fully understood.

    Purpose of the Study:

    • To investigate the role of mitochondrial reactive oxygen species (mROS) in LPS-induced alterations of lipid metabolism in primary cultured frog urinary bladder epithelial cells.

    Main Methods:

    • Primary epithelial cells from frog urinary bladder were cultured.
    • Cells were treated with LPS to induce effects on lipid metabolism.
    • Mitochondrial-targeted antioxidant MitoQ and alpha-tocopherol were used for pretreatment.
    • Lipid droplet formation was visualized using Nile red staining.
    • ROS production and fatty acid oxidation were assessed.

    Main Results:

    • LPS treatment stimulated ROS production and decreased fatty acid oxidation.
    • LPS enhanced intracellular triglyceride deposition and promoted lipid droplet formation.
    • Pretreatment with MitoQ (25 nM, 2 h) nearly abolished all LPS-induced effects.
    • Alpha-tocopherol was ineffective in preventing LPS-induced lipid accumulation.

    Conclusions:

    • Increased mitochondrial ROS (mROS) levels are a critical factor mediating LPS-induced neutral lipid deposition in epithelial cells.
    • Targeting mROS with antioxidants like MitoQ can prevent LPS-induced lipid accumulation.