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Updated: Feb 5, 2026

Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays
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Isolation of Peritoneum-derived Mast Cells and Their Functional Characterization with Ca2+-imaging and Degranulation Assays

Published on: July 4, 2018

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[EFFECT OF MAST CELL DEGRANULATION BLOCKADE ON THE INFLAMMATION OUTCOME IN THE MODEL OF OBSTRUCTIVE LUNG PATHOLOGY]

N A Kuzubova, E S Lebedeva, I V Dvorakovskaya

    Rossiiskii Fiziologicheskii Zhurnal Imeni I.M. Sechenova
    |September 8, 2018
    PubMed

    Insights

    Blocking mast cell degranulation in a rat model of chronic obstructive pulmonary disease reduced inflammation and improved lung tissue structure. This suggests mast cells are a viable therapeutic target for obstructive lung diseases.

    Area of Science:

    • Pulmonary Medicine
    • Immunology
    • Pathology

    Background:

    • Chronic obstructive pulmonary disease (COPD) involves significant lung inflammation and structural damage.
    • Mast cells play a recognized role in mediating inflammatory responses in the airways.

    Purpose of the Study:

    • To investigate the effect of blocking mast cell degranulation on COPD-induced lung inflammation and tissue changes.
    • To evaluate mast cells as a potential therapeutic target in COPD.

    Main Methods:

    • A rat model of COPD was established using chronic nitrogen dioxide exposure.
    • Sodium cromoglicate was administered to block mast cell degranulation.
    • Analysis included lung tissue staining, bronchoalveolar lavage fluid (BALF) analysis, and measurement of inflammatory markers (proteases, TNF-α).

    Main Results:

    • Mast cell degranulation blockade reduced protease release and inflammatory cell influx into the lungs.
    • Tumor necrosis factor-alpha (TNF-α) levels decreased, and interstitial inflammation was absent.
    • Bronchoalveolar epithelium structure and function were restored.

    Conclusions:

    • Mast cells are actively involved in the inflammatory processes of COPD.
    • Inhibiting mast cell degranulation can ameliorate lung inflammation and structural damage in COPD.
    • Targeting mast cells represents a promising therapeutic strategy for obstructive pulmonary diseases.

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