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Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

Effect of Vitamin D on Paclitaxel Efficacy in Triple-negative Breast Cancer Cell Lines

Catherine A Wilhelm¹, Zackary J Clor¹, Jessica L Kelts²

¹Department of Chemistry and Biochemistry, University of Michigan-Flint, Flint, MI, U.S.A.

Anticancer Research

|September 9, 2018

Abstract

Background/Aim:

Triple-negative breast cancer (TNBC) is a highly aggressive breast cancer that disproportionately affects women with darker skin and is often treated with paclitaxel (PTX). Here, the effect of vitamin D on p53-positive DU4475 cells and its ability to decrease the IC₅₀ of PTX in these cells were investigated.

Materials And Methods:

The growth inhibitory effects of vitamin D on DU4475 cells and the effect of PTX plus vitamin D on overall TNBC cell viability was assessed using CellTiter-Glo®.

Results:

Vitamin D increased proliferation of DU4475 cells at low concentrations and lowered the IC₅₀ of PTX. However, it did not change the IC₅₀ of PTX in MDA-MB-231 cells which remained largely viable.

Conclusion:

The effect of vitamin D on DU4475 cell viability was different than in other TNBC cells. The effect of PTX on DU4475 cells was enhanced with vitamin D. MDA-MB-231 cells were relatively resistant to the effects of PTX.

Keywords:

Breast cancer paclitaxel triple-negative breast cancer vitamin D

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