Related Experiment Video
Updated: Feb 5, 2026

Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing
Published on: August 25, 2018
Next-Generation Sequencing Library Preparation for 16S rRNA Microbiome Analysis After Serpin Treatment
Juan Maldonado1,2, Jordan R Yaron3, Liqiang Zhang3
1ASU Genomics Core, Office of Knowledge Enterprise and Development, Arizona State University, Tempe, AZ, USA. juan.maldonadoortiz@asu.edu.
Abstract:
Serine protease inhibitors, serpins, can have profound effects on many systems in the body including immune defense systems. The microbiome, specifically the gut and lung bacterial microbiota, is now known, under some conditions, to alter immune defenses. DNA library preparation for microbiome studies is a procedure that prepares microbial genomic DNA to be sequenced in next-generation sequencing platforms. The construction involves a PCR reaction that will amplify the 16S rRNA gene and will incorporate a specific index and adaptors to the fragments. After confirmation of the product amplification by gel electrophoresis, samples will be later normalized to the same DNA amount of 240 ng. Final concentration of the library is validated by quantitative PCR (qPCR).Here we describe methods to analyze changes in the microbiome after treatment with immune-modulating agents, specifically serpins.
Related Concept Videos
Next-generation Sequencing
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
Sample Preparation for Analysis: Overview
Bulk or large solid samples are typically reduced in size using grinding, crushing, or milling techniques to increase the...
Sample Preparation for Analysis: Advanced Techniques
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Cis-regulatory Sequences
Gene Evolution - Fast or Slow?
In contrast, regions which code...
Sequences

